Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting
Autor: | Toby M. Ward, Niren Murthy, Todd A. Duncombe, Santanu Maity, Mark D. Pegram, Amy E. Herr, Chi-Chih Kang |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Nucleocytoplasmic Transport Proteins Light Receptor ErbB-2 Cell ErbB-2 Engineering Single-cell analysis General Materials Science Extracellular Signal-Regulated MAP Kinases Cancer Tumor Blotting TOR Serine-Threonine Kinases hydrogel pore expansion Hydrogels cancer therapies Blot medicine.anatomical_structure Mechanics of Materials Physical Sciences Self-healing hydrogels Electrophoresis Polyacrylamide Gel Single-Cell Analysis Western Porosity Receptor Electrophoresis Materials science grayscale masks Blotting Western Green Fluorescent Proteins gradient gels Bioengineering Breast Neoplasms western blots Article Cell Line 03 medical and health sciences Cell Line Tumor medicine Humans Nanoscience & Nanotechnology Polyacrylamide Gel Molecular mass Mechanical Engineering Gel electrophoresis of proteins Molecular biology 030104 developmental biology Chemical Sciences Biophysics |
Zdroj: | Duncombe, TA; Kang, CC; Maity, S; Ward, TM; Pegram, MD; Murthy, N; et al.(2016). Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting. Advanced Materials, 28(2), 327-334. doi: 10.1002/adma.201503939. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/99c7h5dg Advanced materials (Deerfield Beach, Fla.), vol 28, iss 2 |
ISSN: | 0935-9648 |
Popis: | © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy. |
Databáze: | OpenAIRE |
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