Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting

Autor: Toby M. Ward, Niren Murthy, Todd A. Duncombe, Santanu Maity, Mark D. Pegram, Amy E. Herr, Chi-Chih Kang
Rok vydání: 2015
Předmět:
0301 basic medicine
Nucleocytoplasmic Transport Proteins
Light
Receptor
ErbB-2
Cell
ErbB-2
Engineering
Single-cell analysis
General Materials Science
Extracellular Signal-Regulated MAP Kinases
Cancer
Tumor
Blotting
TOR Serine-Threonine Kinases
hydrogel pore expansion
Hydrogels
cancer therapies
Blot
medicine.anatomical_structure
Mechanics of Materials
Physical Sciences
Self-healing hydrogels
Electrophoresis
Polyacrylamide Gel
Single-Cell Analysis
Western
Porosity
Receptor
Electrophoresis
Materials science
grayscale masks
Blotting
Western
Green Fluorescent Proteins
gradient gels
Bioengineering
Breast Neoplasms
western blots
Article
Cell Line
03 medical and health sciences
Cell Line
Tumor
medicine
Humans
Nanoscience & Nanotechnology
Polyacrylamide Gel
Molecular mass
Mechanical Engineering
Gel electrophoresis of proteins
Molecular biology
030104 developmental biology
Chemical Sciences
Biophysics
Zdroj: Duncombe, TA; Kang, CC; Maity, S; Ward, TM; Pegram, MD; Murthy, N; et al.(2016). Hydrogel Pore-Size Modulation for Enhanced Single-Cell Western Blotting. Advanced Materials, 28(2), 327-334. doi: 10.1002/adma.201503939. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/99c7h5dg
Advanced materials (Deerfield Beach, Fla.), vol 28, iss 2
ISSN: 0935-9648
Popis: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25-289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light-activated, and acid-expandable hydrogel underpins single-cell protein analysis, here for oncoprotein-related signaling in human breast biopsy.
Databáze: OpenAIRE
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