Guanylin and functional coupling proteins in the hepatobiliary system of rat and guinea pig
| Autor: | Karen Schwabe, Yalcin Cetin |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Histology
SLC4A Proteins Guanylin Anion Transport Proteins Guinea Pigs Cystic Fibrosis Transmembrane Conductance Regulator Biology Antiporters Gastrointestinal Hormones Paracrine signalling Animals Secretion Chloride-Bicarbonate Antiporters Rats Wistar Natriuretic Peptides Molecular Biology Cellular localization Gallbladder Epithelial Cells Cell Biology Apical membrane Transmembrane protein Rats Solute carrier family Cell biology Medical Laboratory Technology Liver Biochemistry Guanylate Cyclase Signal transduction hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | Histochemistry and Cell Biology. 137:589-597 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-012-0927-2 |
| Popis: | Guanylin, a bioactive intestinal peptide, is involved in the cystic fibrosis transmembrane conductance (CFTR)-regulated electrolyte/water secretion in various epithelia. In the present work we report on the expression and cellular localization of guanylin and its affiliated signaling and effector proteins, including guanylate cyclase C (Gucy2c), Proteinkinase GII (Pkrg2), CFTR and the solute carrier family 4, anion exchanger, member 2 (Slc4a2) in the hepatobiliary system of rat and guinea pig. Localization studies in the liver and the gallbladder revealed that guanylin is located in the secretory epithelial cells of bile ducts of the liver and of the gallbladder, while Gucy2c, Pkrg2, CFTR, and Slc4a2 are confined exclusively to the apical membrane of the same epithelial cells. Based on these findings, we assume that guanylin is synthesized as an intrinsic peptide in epithelial cells of the hepatobiliary system and released luminally into the hepatic and cystic bile to regulate electrolyte secretion by a paracrine/luminocrine signaling pathway. |
| Databáze: | OpenAIRE |
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