Whole-genome analysis of EC129, an NDM-5-, CTX-M-14-, OXA-10- and MCR-1-co-producing Escherichia coli ST167 strain isolated from Japan
| Autor: | Shuji Tohda, Yoko Nukui, Saki Kanehira, Ryuji Koike, Yuka Shibuya, Yoshibumi Aiso, Makoto Taniguchi, Ryoichi Saito, Alafate Ayibieke, Yoshiro Hadano, Kazunari Sonobe, Jun Nakajima |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) 030106 microbiology Immunology Microbial Sensitivity Tests medicine.disease_cause Microbiology Genome beta-Lactamases 03 medical and health sciences 0302 clinical medicine Japan Drug Resistance Multiple Bacterial Escherichia coli medicine Immunology and Allergy 030212 general & internal medicine Gene Whole genome sequencing Whole Genome Sequencing biology Escherichia coli Proteins Antimicrobial biology.organism_classification Enterobacteriaceae Anti-Bacterial Agents Carbapenem-Resistant Enterobacteriaceae Genes Bacterial Colistin MCR-1 Genome Bacterial medicine.drug |
| Zdroj: | Journal of Global Antimicrobial Resistance. 18:148-150 |
| ISSN: | 2213-7165 |
| Popis: | Objectives The emergence and spread of carbapenemase-producing Enterobacteriaceae is a worldwide concern. This study reports the whole genome sequence of an NDM-5-, CTX-M-14-, OXA-10- and MCR-1-co-producing Escherichia coli sequence type 167 (ST167) multidrug-resistant clinical strain (EC129) isolated from a sputum sample of a hospitalised patient diagnosed with pneumonia. Methods The genome of E. coli EC129 was subjected to next-generation sequencing and reads were assembled. The draft genome was annotated using DDBJ Read Annotation Pipeline DFAST server, followed by subsequent in silico analysis. Results The genome of E. coli ST167 strain EC129 is 5 319 159 bp in length and contains 5022 protein-coding sequences. The blaNDM-5, blaCTX-M-14, blaOXA-10 and mcr-1 genes were detected along with other antimicrobial resistance genes conferring resistance to aminoglycosides, fluoroquinolones, sulfonamides, trimethoprim and tetracyclines. Antimicrobial susceptibility testing revealed that the isolate was resistant to all antimicrobial agents except colistin. Conclusion To our knowledge, this study is the first to report anE. coli ST167 strain co-producing NDM-5, CTX-M-14, OXA-10 and MCR-1 isolated from a sputum sample of an individual with pneumonia in Japan, thus elucidating the molecular characteristics and resistance gene diversity of this strain. |
| Databáze: | OpenAIRE |
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