Relationship between proliferation and glucose metabolism in primary cultures of rabbit proximal tubules
| Autor: | R. L. Tannen, Ming Jer Tang |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Physiology Pyruvate Kinase Biology Carbohydrate metabolism Kidney Tubules Proximal Internal medicine medicine Animals Glycolysis Cells Cultured chemistry.chemical_classification Kidney Lagomorpha Cell growth Methylglucosides Biological Transport Cell Biology biology.organism_classification Kinetics Glucose medicine.anatomical_structure Endocrinology Enzyme chemistry Cell culture Lactates 3-O-Methylglucose Phosphoenolpyruvate Carboxykinase (GTP) Rabbits Cell Division Pyruvate kinase |
| Zdroj: | American Journal of Physiology-Cell Physiology. 259:C455-C461 |
| ISSN: | 1522-1563 0363-6143 |
| Popis: | Primary cultures of rabbit proximal tubules, which revert to a glycolytic profile as reflected by increased activity of pyruvate kinase (PK) paralleled by increased glucose consumption and lactate formation, were utilized to explore the relationship between glycolytic metabolism and proliferation. Tubules placed in serum-free, hormonally defined Dulbecco's modified Eagle's medium with 5 mM glucose exhibited logarithmic growth beginning on day 3 in culture. The increase in PK activity lagged approximately 1 day behind, suggesting that the reversion to glycolysis is a consequence of rather than a prerequisite for cellular proliferation. Tubules cultured in 0.5 mM as contrasted with 25 mM glucose exhibited heightened proliferation reflected by an increase in protein content and cell number on day 5 in culture. The heightened proliferation was accompanied by increased PK activity. On day 9, after confluency had been achieved, no differences in protein content or PK activity were detected between tubules cultured in different glucose concentrations. These findings indicate that a low glucose concentration is mitogenic for renal proximal tubules and that the proliferative process in some fashion up-regulates the activity of the glycolytic enzyme PK. Furthermore, because accelerated growth proceeds in the presence of glucose restriction, the energy from glycolysis is not required for the proliferative process. |
| Databáze: | OpenAIRE |
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