Enhancing azithromycin antibacterial activity by encapsulation in liposomes/liposomal-N-acetylcysteine formulations against resistant clinical strains of Escherichia coli
| Autor: | Abdulrahman Al-Shehri, Thamer H. Alenazi, Zeyad Alehaideb, Reem E. Alarfaj, Saad M. Al Tamimi, Maaged A. Akiel, Shokran A. Aljihani, Turki M. Albakr, Alaa Eldeen B. Yassin, Majed Halwani, Saad M. Alyahya, Ahmed J. Al-Fahad, Majed F. Alghoribi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine medicine.drug_class Multi-drug resistance bacteria Antibiotics Azithromycin medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences Nanoparticle medicine Escherichia coli lcsh:QH301-705.5 Liposome biology Chemistry Biofilm E. coli biology.organism_classification Enterobacteriaceae N-acetylcysteine 030104 developmental biology lcsh:Biology (General) Liposomes Original Article General Agricultural and Biological Sciences Antibacterial activity Bacteria 010606 plant biology & botany medicine.drug |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 11, Pp 3065-3071 (2020) Saudi Journal of Biological Sciences |
| Popis: | E. coli is an Enterobacteriaceae that could develop resistance to various antibiotics and become a multi-drug resistant (MDR) bacterium. Options for treating MDR E. coli are limited and the pipeline is somewhat dry when it comes to antibiotics for MDR bacteria, so we aimed to explore more options to help in treating MDR E. coli. The purpose of this study is to examine the synergistic effect of a liposomal formulations of co-encapsulated azithromycin and N-acetylcysteine against E. coli. Liposomal azithromycin (LA) and liposomal azithromycin/N-acetylcysteine (LAN) were compared to free azithromycin. A broth dilution was used to measure the MIC and MBC of both formulations. The biofilm reduction activity, thermal stability measurements, stability studies, and cell toxicity analysis were performed. LA and LAN effectively reduced the MIC of E. coli SA10 strain, to 3 μg/ml and 2.5 μg/ml respectively. LAN at 1 × MIC recorded a 93.22% effectiveness in reducing an E. coli SA10 biofilm. The LA and LAN formulations were also structurally stable to 212 ± 2 °C and 198 ± 3 °C, respectively. In biological conditions, the formulations were largely stable in PBS conditions; however, they illustrated limited stability in sputum and plasma. We conclude that the formulation presented could be a promising therapy for E. coli resistance circumstances, providing the stability conditions have been enhanced. |
| Databáze: | OpenAIRE |
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