| Autor: |
Lingam, Swathi, Liu, Zengping, Yang, Binxia, Wong, Wendy, Parikh, Bhav Harshad, Ong, Jun Yi, Goh, Debbie, Wong, Daniel Soo Lin, Tan, Queenie Shu Woon, Tan, Gavin S. W., Holder, Graham E., Regha, Kakkad, Barathi, Veluchamy Amutha, Hunziker, Walter, Lingam, Gopal, Zeng, Xianmin, Su, Xinyi |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.15568518 |
| Popis: |
Additional file 1: Table S1: List of primary antibodies used in the study. figure S1: Estimation of GFP expression by FACS. After lentivirus infection, 95% of the RPC cells expressed GFP (left panel). There was no GFP in the non-infection control (right panel). figure S2: Controls for GFP, SC121 and AMA staining. (A-D) Secondary antibody only controls for Alexa 488 (B), Alexa 568 (C) and Alexa 647 (D). The background for all secondary antibodies appears to be high. (E-H) GFP, SC121 and anti-human mitochondrial antibody staining in non-operated NHP retina. None of the antibodies specifically stained anything in the non-operated control retina. The brightness/ contrast was artificially increased to identify any weakly stained cells. Hoechst 33342 was used to stain the INL and ONL layers (A, E). Scale bar 10 µm. figure S3: Cross reactivity of human lamin B2 (hLMNB2) antibody with the NHP retina. Tissue sections collected from NHP eyes post-photoreceptor precursor transplantation were stained with the human marker SC121 (B) and the human specific nuclear marker lamin B2 (C). The human nuclear marker antibody showed high cross-reactivity with the NHP tissue (C, D). Nuclei were stained with Hoechst 33,342 (H33342). Scale bar: 10 µm. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
