Susceptibility of mouse mammary glands to murine gammaherpesvirus 72 (MHV-72) infection: evidence of MHV-72 transmission via breast milk
| Autor: | Hana Raslova, Marcela Kúdelová, J. Matis, Alain Sarasin, Monique Berebbi, Július Rajčáni |
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| Rok vydání: | 2001 |
| Předmět: |
Time Factors
viruses Mammary gland Mice Nude medicine.disease_cause Microbiology Polymerase Chain Reaction Herpesviridae Virus Salivary Glands Pathogenesis Mice Gammaherpesvirinae Pregnancy medicine Animals Breast Lung Mice Inbred BALB C biology Herpesviridae Infections biology.organism_classification Virology Epstein–Barr virus Epithelium Infectious Disease Transmission Vertical Animals Suckling Virus Latency Disease Models Animal Infectious Diseases medicine.anatomical_structure Lymphatic system Milk DNA Viral Female Lymph Nodes Immunocompetence |
| Zdroj: | Microbial pathogenesis. 31(2) |
| ISSN: | 0882-4010 |
| Popis: | Murine gammaherpesvirus 72 (MHV-72) is a virus of wild rodents and serves as a convenient small animal model to understand the pathogenesis of Epstein-Barr virus (EBV) and human herpesvirus 8 (HHV8) infection. In laboratory mice MHV-72 causes an acute infection of lung epithelial cells and establishes the latency in B lymphocytes. In this study, we investigated athymic nude and immunocompetent mice for distribution of virus in organs after infection with MHV-72. Ten days following subcutaneous dorsal injection of nude mice, virus replicated in lungs, lymphoid organs, salivary glands and also in mammary glands. The virus titre decreased by day 21 post-infection in former tissues, but increased in mammary glands. Presence of virus DNA sequences was detected in the lymphoid and non-lymphoid tissues until the death of the animals (about 1 month post-infection). Infection of immunocompetent mice with MHV-72 induced replication of virus up to 42 days post-infection in mammary glands reaching the highest level of infectious virus at day 8 post-infection. These data show that there is latent infection in mice never detected before. Moreover, virus DNA was detected using nested PCR (by amplification of a portion of gp150 gene sequence) in the mammary glands and the milk of mouse mothers infected with MHV-72 2 days before delivery. We demonstrated the presence of virus DNA also in the milk removed from the stomach of non-infected newborn mice, which were nourished by infected mothers (wet-nurses) for 1 or 2 days. The failure to detect the virus DNA in newborn mice lungs confirmed that they did not become infected from wet-nurses by the intranasal route. This suggests that MHV may be naturally transmitted to newborn mice via breast milk. |
| Databáze: | OpenAIRE |
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