X-tox: An atypical defensin derived family of immune-related proteins specific to Lepidoptera
| Autor: | Michel Brehélin, Jean-Michel Escoubas, Christopher W. Wheat, Yvan Boublik, François Cousserans, Pierre-Alain Girard, Anne-Nathalie Volkoff |
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| Přispěvatelé: | Ecologie microbienne des insectes et interactions hôte-pathogène (EMIP), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), University of Helsinki, Biology Department, Biologie Intégrative et Virologie des Insectes [Univ. de Montpellier II] (BIVI) |
| Rok vydání: | 2008 |
| Předmět: |
DEFENSIN-DERIVED
animal structures Protein family Molecular Sequence Data Immunology Antimicrobial peptides Sf9 Spodoptera Cell Line Defensins Lepidoptera genitalia 03 medical and health sciences fluids and secretions 0302 clinical medicine Tandem repeat Animals Amino Acid Sequence Defensin 030304 developmental biology Genetics 0303 health sciences biology fungi biology.organism_classification Virology LEPIDOPTERA Recombinant Proteins Beta defensin Tandem Repeat Sequences [SDV.IMM]Life Sciences [q-bio]/Immunology Insect Proteins bacteria INSECT IMMUNITY IMMUNE-RELATED PROTEIN HEMOCYTE 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental and Comparative Immunology Developmental and Comparative Immunology, Elsevier, 2008, 32 (5), pp.575-584. ⟨10.1016/j.dci.2007.09.004⟩ |
| ISSN: | 0145-305X |
| Popis: | We report here the isolation in Spodoptera frugiperda (Lepidoptera) of an immune-related protein (hereafter named Spod-11-tox), characterized by imperfectly conserved tandem repeats of 11 cysteine-stabitized alpha beta motifs (CS-alpha beta), the structural scaffold characteristic of invertebrate defensins and scorpion toxins. Spod-11-tox orthologs were only found in Lepidopteran species, suggesting that this new protein family (named X-tox) is specific to this insect order. Moreover, phylogenetic analysis suggests that X-tox proteins represent a new class of proteins restricted to Lepidoptera and likely derived from Lepidopteran defensins. In S. frugiperda, analysis of gene expression revealed that spod-11-tox is rapidly induced by infection. However, and conversely to what is known for most insect antimicrobial peptides (AMP), spod-11-tox is mainly expressed in blood cells. Moreover, recombinant Spod-11-tox produced in the Sf9 cell line does not show any antimicrobial activity. Altogether, these results suggest that although X-tox proteins are derived from defensins, they may play a different and stilt unknown rote in Lepidoptera immune response |
| Databáze: | OpenAIRE |
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