Analysis of a Putative Late Domain Using an Ebola Virus Transcription and Replication-Competent Virus-Like Particle System
| Autor: | Lisa Wendt, Lennart Kämper, Thomas Hoenen, Marie Luisa Schmidt, Thomas C. Mettenleiter |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Transcription Genetic viruses Viral budding Amino Acid Motifs 030106 microbiology Biology Virus Replication medicine.disease_cause Cell Line 03 medical and health sciences VP40 Transcription (biology) medicine Humans Immunology and Allergy Amino Acid Sequence Viral shedding Virus Release chemistry.chemical_classification Budding Viral matrix protein Ebola virus Viral Core Proteins Virion Hemorrhagic Fever Ebola Ebolavirus Cell biology Amino acid HEK293 Cells Nucleoproteins 030104 developmental biology Infectious Diseases chemistry Host-Pathogen Interactions |
| Zdroj: | The Journal of Infectious Diseases. 218:S355-S359 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | The Ebola virus (EBOV) matrix protein VP40 drives budding of virions and encodes 2 overlapping late domain motifs at amino acid positions 7-13 (PTAPPEY). However, these motifs are not absolutely essential for replication in cell culture, and recently a potential third late domain motif (YPx(6)I) was proposed at amino acid positions 18-26 of VP40. To analyze the importance of this motif in viral budding, we used a transcription and replication-competent virus-like particle system. Using this system, we show that this motif does not contribute to EBOV budding or particle propagation. |
| Databáze: | OpenAIRE |
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