Gradients in cytoplasmic calcium concentration ([Ca2+]i) in migrating human umbilical vein endothelial cells (HUVECs) stimulated by shear-stress
| Autor: | Masato Sakon, Hideo Ariyoshi, Tomio Kawasaki, Yasuhisa Aono, Morito Monden, Norihide Yoshikawa |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Intracellular Fluid
Cytoplasm Umbilical Veins Endothelium Cations Divalent chemistry.chemical_element Calcium Biology General Biochemistry Genetics and Molecular Biology Umbilical vein Adenosine Triphosphate Cell Movement Nickel medicine Humans Channel blocker General Pharmacology Toxicology and Pharmaceutics Cells Cultured Fluorescent Dyes Aniline Compounds Microscopy Confocal Cell migration General Medicine Cell biology medicine.anatomical_structure Xanthenes chemistry Endothelium Vascular Stress Mechanical Lamellipodium Intracellular |
| Zdroj: | Life Sciences. 65:2643-2651 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/s0024-3205(99)00533-0 |
| Popis: | Using a parallel-plate flow-chamber and confocal laser scanning microscopy (CLSM), we studied the distribution and temporal changes in intracellular Ca2+ concentration ([Ca2+]i) in migrating HUVECs stimulated by shear-stress. In the presence or absence of ATP, shear-stress (10 dyne/cm2) caused morphological change and migration of individual HUVECs in the random direction. After 120 minute exposure to shear-stress, 70% of the cells migrated in the direction of flow, whereas, as many as 30% of the cells migrated to the upstream against flow. A nonspecific plasma membrane Ca2+ channel blocker, Ni2+, abolished such responses markedly, suggesting that Ca2+ influx may be essential for shear-stress dependent morphological change and migration of HUVECs. Analysis of [Ca2+]i distribution revealed the appearance of localized [Ca2+]i elevation inside lamellipodium formed in the direction of cell migration. The localized rise in [Ca2+]i might be closely related with morphological change to regulate the direction of cell migration induced by shear-stress. |
| Databáze: | OpenAIRE |
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