An ECM-Bound, PDGF-like Growth Factor and a TGF-α-like Growth Factor Are Required for Gastrulation and Spiculogenesis in theLytechinusEmbryo
| Autor: | Jenny M. George, Diane C. Shakes, Ravi K. Ramachandran, Venkatesh Govindarajan, Craig R. Tomlinson |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_treatment
Molecular Sequence Data medicine Extracellular Animals Humans Amino Acid Sequence Molecular Biology Cells Cultured Platelet-Derived Growth Factor Extracellular Matrix Proteins biology Growth factor Cell Differentiation Lytechinus Gastrula Cell Biology Transforming Growth Factor alpha Blastula biology.organism_classification Gastrulation Biochemistry Sea Urchins embryonic structures biology.protein Platelet-derived growth factor receptor Heparan sulfate proteoglycan binding Signal Transduction Developmental Biology Transforming growth factor |
| Zdroj: | Developmental Biology. 172:541-551 |
| ISSN: | 0012-1606 |
| Popis: | Growth factors and the extracellular matrix have been shown to fulfill vital developmental roles in many embryonic systems. Our hypothesis is that a developmental role played by the extracellular matrix in sea urchins may be the binding of a PDGF-like growth factor to promote signaling activity. We report here that anti-human PDGF-B antibodies and anti-human TGF-alpha antibodies immunoprecipitated specific proteins isolated from Lytechinus embryos. Addition of these antibodies to Lytechinus embryos inhibited gastrulation and spiculogenesis. The embryos are sensitive to the antibodies from the four-cell through the hatching blastula stages, which suggests that the TGF-alpha-like and PDGF-like ligands are required for the early differentiation of the gut and spicules. We present evidence that the PDGF-like growth factor depends on the extracellular matrix for signaling activity. Synthetic peptides representing the heparan sulfate proteoglycan binding sequence on human PDGF-B were added to Lytechinus embryo cultures to compete for binding sites with the endogenous PDGF-like growth factor. The experimental peptide inhibited gastrulation and caused radially arranged multiple spicules to form. Development was unaffected by a control peptide. These studies support our hypothesis and suggest that TGF-alpha-like and PDGF-like growth factors induce signaling events required for sea urchin gastrulation and spiculogenesis and suggest that an extracellular matrix-associated PDGF-like growth factor is involved in differentiation along the oral-aboral axis. |
| Databáze: | OpenAIRE |
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