Ig-reactive CD4+CD25+ T cells from toterized (New Zealand black × New Zealand white)F1 mice suppress in vitro production of antibodies to DNA
| Autor: | Fanny M. Ebling, Antonio La Cava, Bevra H. Hahn |
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| Přispěvatelé: | La Cava, A., Ebling, F. M., Hahn, B. H. |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male T cell Antibodie Immunology Population In Vitro Techniques Antibodies Immune tolerance Mice medicine Immune Tolerance Immunology and Allergy Animals IL-2 receptor education Receptor MHC class II education.field_of_study biology Animal In Vitro Technique Receptors Interleukin-2 DNA In vitro medicine.anatomical_structure CD4-Positive T-Lymphocyte Peptide biology.protein Female Antibody Peptides |
| Popis: | We have recently shown that tolerogenic administration of an artificial peptide (pConsensus) that is based on sequences within the VH regions of several murine anti-dsDNA Ig delays appearance of autoantibodies in female (New Zealand Black (NZB) × New Zealand White (NZW))F1 (NZB/W F1) mice and significantly prolongs their survival. The aim of this study was to characterize the T cell population(s) involved in pConsensus-induced down-regulation of autoimmune responses in tolerized NZB/W F1 mice. Using MHC class II dimers loaded with tolerogenic peptide, we found that pCons favored expansion of peptide-reactive CD4+CD25+ regulatory T cells (TR) that inhibited in vitro production of anti-dsDNA Ab-forming cells. Suppression by TR was abrogated by the presence in culture of Ab to glucocorticoid-induced TNFR family member 18 or to TGFβ latency-associated protein. These findings suggest possible relevance of Ag specificity in the mechanism of TR-mediated immune tolerance to Ig-derived peptides in NZB/W F1 mice. |
| Databáze: | OpenAIRE |
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