Establishment of primary cell culture from the temperate symbiotic cnidarian, Anemonia viridis
| Autor: | Diane Dall’Osso, Stéphanie Barnay-Verdier, Pierre-Laurent Merle, Nathalie Joli, Thamilla Zamoum, Juliette Olivre, Paola Furla, Fabrice Priouzeau |
|---|---|
| Přispěvatelé: | Symbiose Marine (SM), Evolution Paris Seine, Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Cnidaria Tentacle Clinical Biochemistry Biomedical Engineering Bioengineering Sea anemone Cnidarian 01 natural sciences Article Anemonia 03 medical and health sciences Symbiosis Botany Primary cell culture 030304 developmental biology 0303 health sciences biology 010604 marine biology & hydrobiology Regeneration (biology) [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] Cell Biology biology.organism_classification Cell biology Cell culture Zooxanthellae Biotechnology |
| Zdroj: | Cytotechnology Cytotechnology, 2013, 65 (5), pp.697-704. ⟨10.1007/s10616-013-9566-2⟩ Cytotechnology, Springer Verlag, 2013, 65 (5), pp.697-704. ⟨10.1007/s10616-013-9566-2⟩ |
| ISSN: | 0920-9069 1573-0778 |
| DOI: | 10.1007/s10616-013-9566-2⟩ |
| Popis: | International audience; The temperate symbiotic sea anemone Anemonia viridis, a member of the Cnidaria phylum, is a relevant experimental model to investigate the molecular and cellular events involved in the preservation or in the rupture of the symbiosis between the animal cells and their symbiotic microalgae, commonly named zooxanthellae. In order to increase research tools for this model, we developed a primary culture from A. viridis animal cells. By adapting enzymatic dissociation protocols, we isolated animal host cells from a whole tentacle in regeneration state. Each plating resulted in a heterogeneous primary culture consisted of free zooxanthellae and many regular, small rounded and adherent cells (of 3-5 mu m diameter). Molecular analyses conducted on primary cultures, maintained for 2 weeks, confirmed a specific signature of A. viridis cells. Further serial dilutions and micromanipulation allowed us to obtain homogenous primary cultures of the small rounded cells, corresponding to A. viridis ``epithelial-like cells''. The maintenance and the propagation over a 4 weeks period of primary cells provide, for in vitro cnidarian studies, a preliminary step for further investigations on cnidarian cellular pathways notably in regard to symbiosis interactions. |
| Databáze: | OpenAIRE |
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