Blood platelet RNA enables the detection of multiple sclerosis
| Autor: | Bakhos A. Tannous, Myron G. Best, Adrienne Vancura, Charlotte E. Teunissen, Sjors G J G In 't Veld, Eva M.M. Strijbis, Joep Killestein, Nik Sol, Thomas Wurdinger, Farrah J. Mateen, Cyra E. Leurs, Markus W. Schweiger |
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| Přispěvatelé: | Neurosurgery, Neurology, Laboratory Medicine, Amsterdam Neuroscience - Neuroinfection & -inflammation, Amsterdam Neuroscience - Neurodegeneration, AII - Cancer immunology, AII - Inflammatory diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty business.industry Multiple sclerosis RNA medicine.disease Original Research Paper 03 medical and health sciences Cellular and Molecular Neuroscience 030104 developmental biology 0302 clinical medicine Cerebrospinal fluid platelets diagnostics biomarker Medicine Biomarker (medicine) Platelet Neurology (clinical) business 030217 neurology & neurosurgery |
| Zdroj: | Multiple sclerosis journal-experimental, translational and clinical, 6(3). SAGE Publications Inc. Sol, N, Leurs, C E, Veld, S GJG I, Strijbis, E M, Vancura, A, Schweiger, M W, Teunissen, C E, Mateen, F J, Tannous, B A, Best, M G, Würdinger, T & Killestein, J 2020, ' Blood platelet RNA enables the detection of multiple sclerosis ', Multiple sclerosis journal-experimental, translational and clinical, vol. 6, no. 3 . https://doi.org/10.1177/2055217320946784 Multiple Sclerosis Journal-Experimental, Translational and Clinical |
| ISSN: | 2055-2173 |
| Popis: | BackgroundIn multiple sclerosis (MS), clinical assessment, MRI and cerebrospinal fluid are important in the diagnostic process. However, no blood biomarker has been confirmed as a useful tool in the diagnostic work-up.ObjectivesBlood platelets contain a rich spliced mRNA repertoire that can alter during megakaryocyte development but also during platelet formation and platelet circulation. In this proof of concept study, we evaluate the diagnostic potential of spliced blood platelet RNA for the detection of MS.MethodsWe isolated and sequenced platelet RNA of blood samples obtained from 57 MS patients and 66 age- and gender-matched healthy controls (HCs). 60% was used to develop a particle swarm-optimized (PSO) support vector machine classification algorithm. The remaining 40% served as an independent validation series.ResultsIn total, 1249 RNAs with differential spliced junction expression levels were identified between platelets of MS patients as compared to HCs, including EPSTI1, IFI6, and RPS6KA3, in line with reported inflammatory signatures in the blood of MS patients. The RNAs were subsequently used as input for a MS classifier, capable of detecting MS with 80% accuracy in the independent validation series.ConclusionsSpliced platelet RNA may enable the blood-based diagnosis of MS, warranting large-scale validation. |
| Databáze: | OpenAIRE |
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