Molecular detection of cosaviruses in a patient with acute flaccid paralysis and in sewage samples in Germany
| Autor: | Victor M. Corman, Marcus Engler, Jörg Hofmann, Christian Drosten, H.C. Selinka, Terry Jones, Joanna Schneider, S. Diedrich, Sindy Böttcher |
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| Rok vydání: | 2020 |
| Předmět: |
Acute flaccid paralysis
Cancer Research medicine.medical_specialty Veterinary medicine Sewage Picornaviridae Biology 03 medical and health sciences Feces Virology Germany Epidemiology medicine Humans Paralysis Phylogeny 030304 developmental biology 0303 health sciences Picornaviridae Infections 030306 microbiology business.industry Phylogenetic study Diagnostic test Neuromuscular Diseases Myelitis Pathogenicity Infectious Diseases Child Preschool Central Nervous System Viral Diseases business |
| Zdroj: | Virus research. 297 |
| ISSN: | 1872-7492 |
| Popis: | Cosaviruses (CoSV) were first identified in stool samples collected from non-polio acute flaccid paralysis (AFP) cases and their healthy contacts in Pakistan in 2003. The clinical importance of CoSV remains unclear as data on epidemiology are scarce and no routine diagnostic testing is done. In this study, we characterized human CoSV (HCoSV) in a child with non-polio AFP and in sewage samples collected in Berlin, Germany. Using unbiased high-throughput sequencing and specific PCR, we characterized a HCoSV-D in stool samples of a three-year-old child hospitalized in Germany with non-polio AFP and travel history to Pakistan. The shedding pattern and absence of other relevant pathogens suggests that HCoSV-D may have been involved in the genesis of AFP. The HCoSV-RNA concentration was high, with 2.57 × 106 copies per mL fecal/suspension, decreasing in follow-up samples. To investigate the possibility of local circulation of HCoSV, we screened Berlin sewage samples collected between 2013 and 2018. Molecular testing of sewage samples has shown the presence of CoSV in several parts of the world, but until now not in Germany. Of our sewage samples, 54.3 % were positive for CoSV, with up to three viral species identified in samples. Phylogenetically, the German sequences clustered intermixed with sequences obtained globally. Together, these findings emphasize the need for further clinical, epidemiological, environmental, pathogenicity and phylogenetic studies of HCoSV. |
| Databáze: | OpenAIRE |
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