Can sterilization methods influence surface properties of resin composites? A purpose for previewing bias in laboratory bacterial adhesion tests

Autor: Letícia Virgínia de Freitas Chaves, Rodolfo Xavier de Sousa-Lima, Lucas José de Azevedo Silva, Boniek Castillo Dutra Borges, Roberta Caroline Brushi Alonso, Kaiza de Sousa Santos
Rok vydání: 2021
Předmět:
Zdroj: Microscopy Research and Technique. 85:1101-1107
ISSN: 1097-0029
1059-910X
Popis: This study aimed to evaluate the influence of sterilization methods on conventional and bulk-fill resin composites' (BFRCs) surface properties in an attempt to preview bias in laboratory bacterial adhesion tests. Two regular viscosity conventional resin composites [Filtek Z350 XT™ (Z350) and IPS Empress Direct™ (ED)] and two regular viscosity BFRCs [Filtek Bulk Fill™ (FILT) and Tetric N-Ceram Bulk Fill IVA™ (TBF)] were used. The materials were characterized by Scanning Electron Microscopy (SEM), surface roughness (SR), and wettability (W) after sterilization with hydrogen peroxide gas plasma (HPGP) and steam sterilization (SS). Nonsterilized samples served as a control group (n = 5). Statistical analysis was performed using two-way analysis of variance (ANOVA) and Tukey post hoc test (p 0.05). For SR, there were no statistically significant differences among the groups (p .05). SS method decreased the contact angle for FILT and Z350 (p .01). The SS promoted more exposition of filler particles, while the HPGP method did not alter the tested materials' morphology. Therefore, sterilization methods affected the resin composites tested selectively. HPGP seems to be the most recommended method to sterilize the tested resin composites before laboratory bacterial adhesion tests.
Databáze: OpenAIRE
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