3D chitinous scaffolds derived from cultivated marine demosponge Aplysina aerophoba for tissue engineering approaches based on human mesenchymal stromal cells
| Autor: | Anja Lode, Sophie Brüggemeier, Mirko Djurovic, Sergey Rodin, Vasilii V. Bazhenov, Birgit Glasmacher, Andre Ehrlich, Hermann Ehrlich, Michael Gelinsky, Dmitriy N. Tarusin, Alexander Y. Petrenko, Vitalii Mutsenko, Allison L. Stelling, Ashwini Rahul Akkineni, Kathleen Schütz, Stephan Meschke, Rajko Martinović, Elke Gossla, Heike Meißner, Olena Rogulska, Alexander G. Tonevitsky, Sergey Nikulin, Slavica Petović |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Survival Cellular differentiation Chitin 02 engineering and technology Biochemistry 03 medical and health sciences chemistry.chemical_compound Tissue engineering Osteogenesis Structural Biology Animals Humans Molecular Biology Cells Cultured Cell Proliferation Adipogenesis Tissue Engineering Tissue Scaffolds Chemistry Cell growth Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Anatomy 021001 nanoscience & nanotechnology Chondrogenesis In vitro Porifera Cell biology 030104 developmental biology 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 104:1966-1974 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.03.116 |
| Popis: | The recently discovered chitin-based scaffolds derived from poriferans have the necessary prosperities for potential use in tissue engineering. Among the various demosponges of the Verongida order, Aplysina aerophoba is an attractive target for more in-depth investigations, as it is a renewable source of unique 3D microporous chitinous scaffolds. We found these chitinous scaffolds were cytocompatible and supported attachment, growth and proliferation of human mesenchymal stromal cells (hMSCs) in vitro. Cultivation of hMSCs on the scaffolds for 7days resulted in a two-fold increase in their metabolic activity, indicating increased cell numbers. Cells cultured onto chitin scaffolds in differentiation media were able to differentiate into the chondrogenic, adipogenic and osteogenic lineages, respectively. These results indicate A. aerophoba is a novel source of chitin scaffolds to futher hMSCs-based tissue engineering strategies. |
| Databáze: | OpenAIRE |
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