Identification of three genes up-regulated in PU.1 rescued monocytic precursor cells
| Autor: | Gregory W. Henkel, Richard A. Maki, Scott R. McKercher |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Macrophage colony-stimulating factor Genetic Vectors Immunology Tetraspanin 25 Biology Monocytes Antigens CD Transduction Genetic Proto-Oncogene Proteins Precursor cell Null cell medicine Animals Calgranulin B Immunology and Allergy Macrophage RNA Messenger Receptors Immunologic Transcription factor Adaptor Proteins Signal Transducing Regulation of gene expression Macrophage Colony-Stimulating Factor Gene Expression Regulation Developmental Granulocyte-Macrophage Colony-Stimulating Factor Membrane Proteins Nucleic Acid Hybridization Cell Differentiation General Medicine Blotting Northern Molecular biology Up-Regulation Retroviridae Granulocyte macrophage colony-stimulating factor Suppression subtractive hybridization Trans-Activators RNA medicine.drug |
| Zdroj: | International Immunology. 14:723-732 |
| ISSN: | 1460-2377 |
| DOI: | 10.1093/intimm/dxf040 |
| Popis: | The requirement of the transcription factor PU.1 for macrophage development has been well documented. However, the target genes regulated by PU.1 controlling macrophage maturation are not known. A granulocyte macrophage colony stimulating factor (GM-CSF)-dependent PU.1 null monocytic precursor cell was stably transduced with a PU.1-expressing retrovirus. The expression of PU.1 altered the surface expression of a few proteins expressed on monocytes; these cells, however, remained GM-CSF dependent and maintained an immature phenotype. In contrast to the PU.1 null cells, the cells expressing PU.1 responded to macrophage colony stimulating factor (M-CSF) with subsequent development into mature macrophages. Using suppressive subtractive hybridization between the PU.1 null and immature PU.1 rescued cells, three genes, MRP-14, Dap12 and CD53, were found expressed in the rescued cells, but not in the PU.1 null cells. In addition, these genes were modulated during M-CSF-induced maturation of the PU.1 rescued cells. The PU.1 null and rescued early monocytic cells provide a useful model to study the role of PU.1 in macrophage development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|