Long Noncoding RNA PTPRG Antisense RNA 1 Reduces Radiosensitivity of Nonsmall Cell Lung Cancer Cells Via Regulating MiR-200c-3p/TCF4
| Autor: | Liangshan Da, Wei Huang, Congjun Zhang, Yanlei Tang, Daowen Jiang, Yanfeng Xi, Rungui Niu, Qiang Ma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Lung Neoplasms Biology NSCLC Models Biological Radiation Tolerance lcsh:RC254-282 03 medical and health sciences miR-200c-3p 0302 clinical medicine Transcription Factor 4 Carcinoma Non-Small-Cell Lung Cell Line Tumor RNA PTPRG-AS1 Biomarkers Tumor Humans Radiosensitivity 3' Untranslated Regions 030304 developmental biology TCF4 0303 health sciences Receptor-Like Protein Tyrosine Phosphatases Class 5 Dose-Response Relationship Radiation lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Long non-coding RNA Antisense RNA Gene Expression Regulation Neoplastic MicroRNAs Oncology radiosensitivity 030220 oncology & carcinogenesis Cancer research Original Article RNA Interference RNA Long Noncoding Non small cell Mir 200c |
| Zdroj: | Technology in Cancer Research & Treatment, Vol 19 (2020) Technology in Cancer Research & Treatment |
| ISSN: | 1533-0338 |
| Popis: | Background: PTPRG antisense RNA 1 has been well-documented to exert an oncogenic role in diverse neoplasms. However, the precise role of PTPRG antisense RNA 1 in regulating radiosensitivity of nonsmall cell lung cancer cells remains largely elusive. Methods: Expression levels of PTPRG antisense RNA 1 and miR-200c-3p in nonsmall cell lung cancer tissues and cells were detected by quantitative real-time polymerase chain reaction, while transcription factor 4 expression was examined by immunohistochemistry and Western blot. After nonsmall cell lung cancer cells were exposed to X-ray with different doses in vitro, Cell Counting Kit -8 assay and colony formation assay were conducted to determine the influence of PTPRG antisense RNA 1 on cell viability. Interaction between miR-200c-3p and PTPRG antisense RNA 1 as well as transcription factor 4 was investigated by dual luciferase reporter assay. Result: In nonsmall cell lung cancer tissues, the expressions of PTPRG antisense RNA 1 and transcription factor 4 were significantly upregulated, whereas the expression of miR-200c-3p was downregulated. It was also proved that PTPRG antisense RNA 1 and 3′-untranslated region of transcription factor 4 can bind to miR-200c-3p. Under X-ray irradiation, overexpressed PTPRG antisense RNA 1 could promote the viability and enhance the radioresistance of nonsmall cell lung cancer cells, and this effect was partially weakened by miR-200c-3p mimics. Transcription factor 4 was identified as a target gene of miR-200c-3p, which could be positively regulated by PTPRG antisense RNA 1. Conclusion: PTPRG antisense RNA 1 reduces the radiosensitivity of nonsmall cell lung cancer cells via modulating miR-200c-3p/TCF4 axis. |
| Databáze: | OpenAIRE |
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