β-Carbolines in rat arcuate nucleus
| Autor: | T.G. Bidder, D.W. Shoemaker, J.T. Cummins |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Male
Nialamide Indoles Chlorpromazine Hypothalamus Analytical chemistry medicine.disease_cause Photochemistry chemistry.chemical_compound medicine Animals Emission spectrum Irradiation Brain Mapping Arcuate nucleus (medulla) Histocytochemistry Lasers General Neuroscience Acetaldehyde Reserpine Fluorescence Rats Spectrometry Fluorescence chemistry Ultraviolet Carbolines medicine.drug |
| Zdroj: | Neuroscience. 3:233-239 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/0306-4522(78)90104-5 |
| Popis: | The arcuate nucleus of the rat exhibits a brilliant yellow-green fluorescence when irradiated in situ with a He-Cd ultraviolet laser microfluorimeter employing 325 nm incident light. Fluorescent emission spectra demonstrated peaks at 445 nm, 470 nm and 520 nm. The latter peak undergoes rapid photodecomposition. This spectral pattern, including the photodecomposition, is characteristic of β-carbolines. The presence of β-carbolines was confirmed by extraction of arcuate nuclei with organic solvents and subsequent thin-layer chromatography. Two compounds were tentatively identified: 6-methoxy-1,2,3,4-tetrahydro-β-carboline and 1-methyl-β-carboline. The effect of treatment of rats with certain pharmacological agents on the arcuate fluorochromes was determined by measuring the fluorescent intensity at 520 nm. Chlorpromazine treatment decreased the fluorescence by 90% while acetaldehyde and 5,6-dihydroxytryptamine produced increases of 40 and 100% respectively. Treatment with nialamide or reserpine did not significantly effect the fluorescence intensity. |
| Databáze: | OpenAIRE |
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