C1'-Branched acyclic nucleoside phosphonates mimicking adenosine monophosphate: Potent inhibitors of Trypanosoma brucei adenine phosphoribosyltransferase
| Autor: | Zlatko Janeba, Alena Zíková, Martina Slapničková, Lenka Poštová Slavětínská, Petr Pachl, Jan Frydrych, Filip Kalčic, Martin Dračínský, Dana Hocková, Eva Doleželová |
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| Rok vydání: | 2021 |
| Předmět: |
Adenosine monophosphate
Trypanosoma brucei brucei Adenine phosphoribosyltransferase Adenine Phosphoribosyltransferase Antiprotozoal Agents Trypanosoma brucei chemistry.chemical_compound Structure-Activity Relationship Parasitic Sensitivity Tests Drug Discovery Nucleotide Enzyme Inhibitors Purine metabolism Nucleotide salvage Pharmacology chemistry.chemical_classification biology Dose-Response Relationship Drug Molecular Structure Acyclic nucleoside Organic Chemistry Nucleosides General Medicine biology.organism_classification Adenosine Monophosphate Enzyme chemistry Biochemistry |
| Zdroj: | European journal of medicinal chemistry. 225 |
| ISSN: | 1768-3254 |
| Popis: | Some pathogens, including parasites of the genus Trypanosoma causing Human and Animal African Trypanosomiases, cannot synthesize purines de novo and they entirely rely on the purine salvage pathway (PSP) for their nucleotide generation. Thus, their PSP enzymes are considered as promising drug targets, sparsely explored so far. Recently, a significant role of acyclic nucleoside phosphonates (ANPs) as inhibitors of key enzymes of PSP, namely of 6-oxopurine phosphoribosyltransferases (PRTs), has been discovered. Herein, we designed and synthesized two series of new ANPs branched at the C1′ position as mimics of adenosine monophosphate. The novel ANPs efficaciously inhibited Trypanosoma brucei adenine PRT (TbrAPRT1) activity in vitro and it was shown that the configuration on the C1′ chiral centre strongly influenced their activity: the (R)-enantiomers proved to be more potent compared to the (S)-enantiomers. Two ANPs, with Ki values of 0.39 μM and 0.57 μM, represent the most potent TbrAPRT1 inhibitors reported to date and they are an important tool to further study purine metabolism in various parasites. |
| Databáze: | OpenAIRE |
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