| Autor: |
Menno Willem Jose Prins, A.M. de Jong, A. van Reenen, Xander J. A. Janssen, L.J. van IJzendoorn |
| Přispěvatelé: |
Molecular Biosensing for Med. Diagnostics |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 373(1-3), 88-93. Elsevier |
| ISSN: |
0927-7757 |
| DOI: |
10.1016/j.colsurfa.2010.10.033 |
| Popis: |
We demonstrate a new probing technique to measure physicochemical interactions between particles and a substrate in a fluid. The technique is based on the measurement of field-induced rotation of individual magnetic particles in contact with the substrate. The parallel measurement of many particles with single-particle resolution gives reliable statistics in a short time. Using this technique, the interaction between streptavidin-coated magnetic particles and a glass substrate is measured for various buffer conditions. Increased binding is quantified for increasing ionic strength and decreasing pH. The results are found to be in agreement with calculations of electrostatic interactions. We also apply the technique to study how binding is reduced by blocking the substrate with albumin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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