Prevalence of verotoxin-producing Escherichia coli (VTEC) in slurry, farmyard manure and sewage sludge in France
| Autor: | E. Serillon, J. Bouvet, B. Tilly, F. Lequerrec, C. Bavai, S. Ray-Gueniot, C. Mazuy-Cruchaudet, M.P. Montet, C. Vernozy-Rozand, Y. Richard |
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| Rok vydání: | 2002 |
| Předmět: |
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Bacterial Serotype Veterinary medicine Sewage Biology Escherichia coli O157 Shiga Toxin 1 Shiga Toxins Polymerase Chain Reaction Shiga Toxin 2 Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound fluids and secretions Shiga-like toxin STX2 Escherichia coli Prevalence Animals Animal Husbandry Adhesins Bacterial Escherichia coli Infections Feces business.industry Escherichia coli Proteins food and beverages General Medicine Manure chemistry VTEC Cattle France Carrier Proteins business Sludge Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 93:473-478 |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1046/j.1365-2672.2002.01706.x |
| Popis: | Aims: The aims of the present study were to determine VTEC prevalence in manure, slurryand sewage sludge in France and to characterize the VTEC strains isolated (virulence genes and serotype). Methods and Results: Seven hundred and fifty-two samples from 55 farmyard manures, 136 bovine and porcine faeces, 114 slurries, 10 composts, and 437 samples from outflows of sewagewastewater treatment plants were analysed. Twenty-four percent contained isolates which were PCR positive for stx gene. Twenty-one VTEC strains were recovered from positive samples by colony hybridization: 76% of them were positive for stx2 gene, 33% for stx1 gene,and 19% for eae gene. One strain belonged to serotype O157:H7 and two others to serogroups O26 and O55, respectively. Conclusions: Some of the VTEC strains isolated from environments in France should be considered as potentially pathogenic for humans. Significance and Impact of the Study: Appropriate handling or use of manure, slurry andsewage sludge is necessary so that contamination of the environment and food by VTEC can be prevented. |
| Databáze: | OpenAIRE |
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