Temporal Alterations in Mitochondrial β-Oxidation and Oxidative Stress Aggravate Chronic Kidney Disease Development in 5/6 Nephrectomy Induced Renal Damage

Autor: José Pedraza-Chaverri, Pedro Rojas-Morales, Alexis Paulina Jiménez-Uribe, Edilia Tapia, Rodrigo Prieto-Carrasco, Sabino Hazael Avila-Rojas, Rogelio Hernández-Pando, Laura G. Sánchez-Lozada, Juan Carlos León-Contreras, Omar Emiliano Aparicio-Trejo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Time Factors
Bioenergetics
β-oxidation impairment
mitochondrial damage
medicine.medical_treatment
Mitochondrion
Kidney
medicine.disease_cause
urologic and male genital diseases
Nephrectomy
lcsh:Chemistry
chemistry.chemical_compound
Postoperative Complications
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
ATP synthase
biology
5/6 nephrectomy
General Medicine
female genital diseases and pregnancy complications
Mitochondria
Computer Science Applications
Disease Progression
Oxidation-Reduction
medicine.medical_specialty
Oxidative phosphorylation
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Oxygen Consumption
Internal medicine
medicine
Animals
Rats
Wistar
Renal Insufficiency
Chronic
Physical and Theoretical Chemistry
Molecular Biology
business.industry
Organic Chemistry
Hemodynamics
medicine.disease
Rats
Oxidative Stress
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
biology.protein
Energy Metabolism
business
Adenosine triphosphate
030217 neurology & neurosurgery
Oxidative stress
chronic kidney disease
Kidney disease
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 18
International Journal of Molecular Sciences, Vol 21, Iss 6512, p 6512 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21186512
Popis: Five-sixths nephrectomy (5/6Nx) model is widely used for studying the mechanisms involved in chronic kidney disease (CKD) progression, a kidney pathology that has increased dramatically in recent years. Mitochondrial impairment is a key mechanism that aggravates CKD progression
however, the information on mitochondrial bioenergetics and redox alterations along a time course in a 5/6Nx model is still limited and in some cases contradictory. Therefore, we performed for the first time a time-course study of mitochondrial alterations by high-resolution respirometry in the 5/6Nx model. Our results show a decrease in mitochondrial &beta
oxidation at early times, as well as a permanent impairment in adenosine triphosphate (ATP) production in CI-linked respiration, a permanent oxidative state in mitochondria and decoupling of these organelles. These pathological alterations are linked to the early decrease in complex I and ATP synthase activities and to the further decrease in complex III activity. Therefore, our results may suggest that mitochondrial bioenergetics impairment is an early event in renal damage, whose persistence in time aggravates CKD development in the 5/6Nx model.
Databáze: OpenAIRE
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