MSP-4, an Antimicrobial Peptide, Induces Apoptosis via Activation of Extrinsic Fas/FasL- and Intrinsic Mitochondria-Mediated Pathways in One Osteosarcoma Cell Line
| Autor: | Shu-Yu Cheng, Ming-Hong Tai, Chien-Wei Feng, Yen-Hsuan Jean, Han-Chun Hung, Nan-Fu Chen, Zhi-Hong Wen, Chung-Chih Tseng, Shi-Ying Huang, Hsiao-Mei Kuo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fas Ligand Protein antimicrobial peptide mitochondria pathways Blotting Western Antimicrobial peptides Pharmaceutical Science Antineoplastic Agents Bone Neoplasms Caspase 3 Caspase 8 Article Fas ligand Fas/FasL pathway 03 medical and health sciences 0302 clinical medicine Anti-Infective Agents Western blot osteosarcoma apoptosis Cell Line Tumor Drug Discovery parasitic diseases medicine Animals Humans fas Receptor Pharmacology Toxicology and Pharmaceutics (miscellaneous) Osteosarcoma medicine.diagnostic_test Chemistry Cichlids Fas receptor medicine.disease Caspase 9 Mitochondria 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis Cancer research Peptides |
| Zdroj: | Marine Drugs; Volume 16; Issue 1; Pages: 8 Marine Drugs |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md16010008 |
| Popis: | Osteosarcoma (OS) is a common malignant bone cancer. The relatively high density of a person’s bone structure means low permeability for drugs, and so finding drugs that can be more effective is important and should not be delayed. MSPs are marine antimicrobial peptides (AMP) and natural compounds extracted from Nile tilapia (Oreochromis niloticus). MSP-4 is a part of the AMPs series, with the advantage of having a molecular weight of about 2.7-kDa and anticancer effects, although the responsible anticancer mechanism is not very clear. The goal of this study is to determine the workings of the mechanism associated with apoptosis resulting from MSP-4 in osteosarcoma MG63 cells. The study showed that MSP-4 significantly induced apoptosis in MG63 cells, with Western blot indicating that MSP-4 induced this apoptosis through an intrinsic pathway and an extrinsic pathway. Thus, a pretreatment system with a particular inhibitor of Z-IETD-FMK (caspase-8 inhibitor) and Z-LEHD-FMK (caspase-9 inhibitor) significantly attenuated the cleavage of caspase-3 and prevented apoptosis. These observations indicate that low concentrations of MSP-4 can help induce the apoptosis of MG63 through a Fas/FasL- and mitochondria-mediated pathway and suggest a potentially innovative alternative to the treatment of human osteosarcoma. |
| Databáze: | OpenAIRE |
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