In situ decellularization of a large animal saccular aneurysm model: sustained inflammation and active aneurysm wall remodeling
| Autor: | Alexei A. Bogdanov, Suresh Gupta, Erin T. Langan, Ajit S. Puri, Christopher M. Raskett, Anita M. Leporati, Aurora Rodríguez-Rodríguez, Peter Caravan, Matthew J. Gounis, Jildaz Caroff, Robert M. King |
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| Rok vydání: | 2020 |
| Předmět: |
In situ
Male Pathology medicine.medical_specialty Inflammation Vascular Remodeling Article 030218 nuclear medicine & medical imaging 03 medical and health sciences Random Allocation 0302 clinical medicine Aneurysm medicine Image Processing Computer-Assisted Animals cardiovascular diseases Decellularization biology medicine.diagnostic_test business.industry Elastase Angiography Digital Subtraction General Medicine Digital subtraction angiography medicine.disease Magnetic Resonance Imaging Disease Models Animal Myeloperoxidase biology.protein cardiovascular system Surgery Female Neurology (clinical) Endothelium Vascular Rabbits medicine.symptom business 030217 neurology & neurosurgery Large animal |
| Zdroj: | J Neurointerv Surg |
| ISSN: | 1759-8486 |
| Popis: | ObjectiveTo investigate in situ decellularization of a large animal model of saccular aneurysm as a strategy for achieving aneurysmal growth and lasting inflammation.Methods18 New Zealand White rabbits were randomized 2:1 to receive endoluminal sodium dodecyl sulfate infusion (SDS, 1% solution, 45 min) following elastase or elastase-only treatment (control). All aneurysms were measured by digital subtraction angiography every 2 weeks. Every 2 weeks, three of the rabbits (two elastase + SDS, one control) underwent MRI, followed by contrast injection with myeloperoxidase (MPO)-sensing contrast agent. MRI was repeated 3 hours after contrast injection and the enhancement ratio (ER) was calculated. Following MRI, aneurysms were explanted and subjected to immunohistopathology.ResultsDuring follow-up MRI, the average ER for SDS-treated animals was 1.63±0.20, compared with 1.01±0.06 for controls (pConclusionsIn situ decellularization of a large animal model of saccular aneurysms reproduces features of unstable aneurysms, such as chronic inflammation (up to 12 weeks) and active aneurysm wall remodeling, leading to continued growth over 8 weeks. |
| Databáze: | OpenAIRE |
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