Phenotypic and Genotypic Heterogeneity among Cultivable Pathogen-Related Oral Spirochetes and Treponema vincentii
| Autor: | G. R. Riviere, K. S. Smith, K. H. Riviere, S. G. Willis |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
Genotype Immunoblotting Treponema vincentii Cross Reactions Biology urologic and male genital diseases Polymerase Chain Reaction law.invention Microbiology stomatognathic system Species Specificity law RNA Ribosomal 16S Humans Treponema Treponema pallidum Ribosomal DNA Polymerase chain reaction DNA Primers Genetics Antigens Bacterial Mouth Base Sequence Antibodies Monoclonal Bacteriology Ribosomal RNA 16S ribosomal RNA biology.organism_classification Spirochaetaceae humanities Bacterial Typing Techniques Culture Media body regions RNA Bacterial Phenotype Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Microbiology. 37:3676-3680 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.37.11.3676-3680.1999 |
| Popis: | Recent findings challenge the assumption that pathogen-related oral spirochetes (PROS) are related to Treponema pallidum. Treponema vincentii , grown in OMIZ-Pat media, cross-reacted with monoclonal antibody H9-2 against T. pallidum , and cultivable PROS had 16S rRNA gene sequences similar to those of T. vincentii (C.-B. Choi, C. Wyss, and U. B. Göbel. J. Clin. Microbiol. 34:1922–1925, 1996). Aims of the present study were to determine whether antigen phenotypes of oral treponemas were influenced by growth conditions and to evaluate the genetic relatedness of cultivable PROS to T. pallidum and T. vincentii . Results show that three T. pallidum monoclonal antibodies (H9-1, H9-2, and F5) cross-reacted with whole cells from four Treponema species grown in modified OMIZ-Pat medium, but not with treponemas grown in NOS medium. Only H9-2 reacted in immunoblots with reduced proteins from cultivable PROS and T. vincentii . Three of five PROS isolates were amplified by T. vincentii -specific PCR, and one was amplified by Treponema medium -specific PCR. None were amplified by T. pallidum -specific PCR. Three of five PROS isolates had 16S ribosomal DNA restriction fragment length polymorphism patterns identical to that of T. vincentii , and the patterns of two isolates resembled that of T. medium . Arbitrarily primed-PCR profiles from whole genomic DNA were distinct among five PROS isolates and two T. vincentii strains. Thus, PROS isolates represent a heterogeneous group of treponemas that share some 16S rRNA gene sequences with T. vincentii and T. medium , but not with T. pallidum . It is proposed that the PROS nomenclature be dropped. |
| Databáze: | OpenAIRE |
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