UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration
| Autor: | Heidi N. Fridolfsson, Marina Meyerzon, Daniel A. Starr, Nina Ly |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Microtubule-associated protein
Nuclear Envelope Dynein Nuclear migration Kinesins macromolecular substances Biology Article 03 medical and health sciences 0302 clinical medicine Microtubule medicine Animals Nuclear protein Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology 030304 developmental biology Cell Nucleus 0303 health sciences KASH proteins Dyneins Membrane Proteins Nuclear Proteins Biological Transport Kinesin Cell Biology Cell biology Transport protein Cell nucleus medicine.anatomical_structure Multiprotein Complexes Dynactin C. elegans Microtubule-Associated Proteins 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Biology. 338(2):237-250 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2009.12.004 |
| Popis: | Nuclei migrate during many events, including fertilization, establishment of polarity, differentiation, and cell division. The Caenorhabditis elegans KASH protein UNC-83 localizes to the outer nuclear membrane where it recruits kinesin-1 to provide the major motor activity required for nuclear migration in embryonic hyp7 cells. Here we show that UNC-83 also recruits two dynein-regulating complexes to the cytoplasmic face of the nucleus that play a regulatory role. One consists of the NudE homolog NUD-2 and the NudF/Lis1/Pac1 homolog LIS-1, and the other includes dynein light chain DLC-1, the BicaudalD homolog BICD-1, and the Egalitarian homologue EGAL-1. Genetic disruption of any member of these two complexes caused nuclear migration defects that were enhanced in some double mutant animals, suggesting that BICD-1 and EGAL-1 function in parallel to NUD-2. Dynein heavy chain mutant animals also had a nuclear migration defect, suggesting these complexes function through dynein. Deletion analysis indicated that independent domains of UNC-83 interact with kinesin and dynein. These data suggest a model where UNC-83 acts as the cargo-specific adaptor between the outer nuclear membrane and the microtubule motors kinesin-1 and dynein. Kinesin-1 functions as the major force generator during nuclear migration, while dynein is involved in regulation of bidirectional transport of the nucleus. |
| Databáze: | OpenAIRE |
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