Relationship of antibody nitrogen to titer obtained in the cercarial antigen slide flocculation test for schistosomiasis
| Autor: | Robert I. Anderson |
|---|---|
| Rok vydání: | 1962 |
| Předmět: |
Nitrogen
Immunology Positive reaction Immunoglobulins chemistry.chemical_element Schistosomiasis Trematode Infections Biology Antibodies Microbiology Antigen-Antibody Reactions Antigen medicine Animals Cercaria Heterophyidae Flocculation test Chromatography Antigen-antibody reactions Flocculation Tests General Medicine medicine.disease Titer Infectious Diseases chemistry biology.protein Parasitology Antibody |
| Zdroj: | Experimental Parasitology. 12:434-440 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(62)90079-6 |
| Popis: | It was possible to absorb all of the serologically reactive antibody from schistosomiasis sera with a single calculated volume of washed, packed, and essentially dry cholesterollecithin crystals coated with cercarial antigen. Micro-Kjeldahl nitrogen determinations performed on the antigen complex and on absorbed antibody-antigen complexes were used to estimate the relationship of antibody nitrogen to titer. The relationship was found to be directly proportional, since a graph of mg N/ml of sera plotted on arithmetic ordinates against the titer formed a straight line. Approximately 2.1 μg of antibody nitrogen is required in the flocculation test to give a minimal positive reaction. A possible practical application of this work is the use of the determination for nitrogen content to standardize control sera for serologic surveys. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|