Soy milk digestion extract inhibits progression of prostate cancer cell growth via regulation of prostate cancer-specific antigen and cell cycle-regulatory genes in human LNCaP cancer cells
| Autor: | Kang Nam Hee, Kyung Chul Choi, Hee‑Chang Shin, Seung Hyun Oh, Yoon Bok Lee, Kyun Hee Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cancer Research medicine.medical_specialty Estrogen receptor Genistein Cell Cycle Proteins Biology Biochemistry 03 medical and health sciences Prostate cancer chemistry.chemical_compound 0302 clinical medicine Prostate Cell Line Tumor Internal medicine LNCaP Genetics medicine Humans RNA Messenger Molecular Biology Cell Proliferation Plant Extracts Cell growth Cancer Prostate-Specific Antigen medicine.disease Antineoplastic Agents Phytogenic Soy Milk Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure Endocrinology Receptors Estrogen Oncology chemistry 030220 oncology & carcinogenesis Cancer cell Cancer research Molecular Medicine Biomarkers |
| Zdroj: | Molecular Medicine Reports. 14:1809-1816 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2016.5408 |
| Popis: | Soy milk, which is produced from whole soybeans, contains a variety of biologically active components. Isoflavones are a class of soy-derived phytoestrogens with beneficial effects, among which genistein (GEN) has been previously indicated to reduce the risk of prostate cancer. The present study evaluated the effects of soy milk digestion extract (SMD) on the progression of prostate cancer via the estrogen receptor (ER)β in human LNCaP prostate cancer cells. To evaluate the effects of SMD (daizein, 1.988 mg/100g, glycitein, 23.537 mg/100 g and GEN, 0.685 mg/100g) on cell proliferation, LNCaP cells were cultured in media containing vehicle (0.1% dimethyl sulfoxide), 17β‑estradiol (E2; 2.7x10‑7 mg/ml), GEN (2.7x10-2 mg/ml) of SMD (total aglycon concentration, 0.79 mg/ml), after which the cell viability was examined using an MTT assay. The cell viability was significantly elevated by E2 (by 45±0.18%), while it was markedly reduced by GEN (73.2±0.03%) or SMD (74.8±0.09%). Semi‑quantitative reverse transcription polymerase chain reaction analysis was performed to assess the mRNA expression levels of target genes, including ERβ, prostate cancer‑specific antigen (PSA) and cell cycle regulators p21, Cyclin D1 and cyclin-dependent kinase (CDK)4. The expression of ERβ was almost completely diminished by E2, whereas it was significantly elevated by SMD. In addition, the expression levels of PSA were considerably reduced by SMD. The expression of p21 was significantly elevated by SMD, while it was markedly reduced by E2. Of note, the expression levels of Cyclin D1 and CDK4 were considerably elevated by E2, while being significantly reduced by GEN and SMD. All of these results indicated that SMD may inhibit the proliferation of human prostate cancer cells via regulating the expression of ERβ, PSA, p21, Cyclin D1 and CDK4 in an ER-dependent manner. |
| Databáze: | OpenAIRE |
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