Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843

Autor: Poonsuk Prasertsan, Maneewan Suwansaard, Wiboon Riansa-ngawong
Rok vydání: 2012
Předmět:
Zdroj: Electronic Journal of Biotechnology, Volume: 15, Issue: 6, Pages: 1-1, Published: 15 NOV 2012
Electronic Journal of Biotechnology v.15 n.6 2012
SciELO Chile
CONICYT Chile
instacron:CONICYT
ISSN: 0717-3458
DOI: 10.2225/vol15-issue6-fulltext-1
Popis: Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO 2 . C. shehatae TISTR5843 was grown in modified yeast extract- malt (YM) medium at 30 ± 2oC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L -1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g L -1 , ethanol yield 0.47 g g -1 ) than those of free cells (ethanol production 10.8 g L -1 , ethanol yield 0.44 g g -1 ) after 36 hrs of cultivation. In contrast, the small size of PPF (sPPF) was selected as a carrier in repeated batch fermentation for cost effectiveness. The ethanol productivity of immobilized yeast cells in repeated batch fermentations was 45.2-51.6% greater than that obtained from batch fermentations. The immobilized cells on sPPF improved the ethanol production and could be reused 4 times with retaining the activity of 93%. In conclusion, PPF is a potential carrier in the immobilization system. The pre-treatment of PPF increases the surface area that enhances cell adsorption and ethanol production by C. shehatae TISTR5843.
Databáze: OpenAIRE
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