Localization of Glucose Oxidase and Catalase Activities in Aspergillus niger
| Autor: | Marten Veenhuis, C.F.B. Witteveen, Jaap Visser |
|---|---|
| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology |
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
DEGLYCOSYLATION
PROTEINS Mycology Laboratorium voor Erfelijkheidsleer Applied Microbiology and Biotechnology Cell membrane chemistry.chemical_compound medicine Life Science Glucose oxidase Polyacrylamide gel electrophoresis chemistry.chemical_classification Ecology biology Aspergillus niger Protoplast biology.organism_classification medicine.anatomical_structure Enzyme chemistry Biochemistry Gluconic acid biology.protein Laboratory of Genetics PEROXISOMES Intracellular Food Science Biotechnology |
| Zdroj: | Applied and environmental microbiology, 58(4), 1190-1194. AMER SOC MICROBIOLOGY Applied and Environmental Microbiology 58 (1992) 4 Applied and Environmental Microbiology, 58(4), 1190-1194 |
| ISSN: | 0099-2240 |
| Popis: | The subcellular localization of glucose oxidase (EC 1.1.3.4) in Aspergillus niger N400 (CBS 120.49) was investigated by (immuno)cytochemical methods. By these methods, the bulk of the enzyme was found to be localized in the cell wall. In addition, four different catalases (EC 1.11.1.6) were demonstrated by nondenaturing polyacrylamide gel electrophoresis of crude extracts of induced and noninduced cells. Comparison of both protoplast and mycelial extracts indicated that, of two constitutive catalases, one is located outside the cell membrane whereas the other is intracellular. Parallel with the induction of glucose oxidase, two other catalases are also induced, one located intracellularly and one located extracellularly. Furthermore, lactonase (EC 3.1.1.17) activity, catalyzing the hydrolysis of glucono-δ-lactone to gluconic acid, was found to be exclusively located outside the cell membrane, indicating that gluconate formation in A. niger occurs extracellularly. |
| Databáze: | OpenAIRE |
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