Silencing activity of 2'-O-methyl modified anti-MDR1 siRNAs with mismatches in the central part of the duplexes
| Autor: | Elena L. Chernolovskaya, Valentin V. Vlassov, Marina A. Zenkova, Natalya S. Petrova, Alya G. Venyaminova, Mariya I. Meschaninova |
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| Rok vydání: | 2011 |
| Předmět: |
Small interfering RNA
ATP Binding Cassette Transporter Subfamily B Base Pair Mismatch Biophysics MDR1 Thermodynamic stability Biochemistry RNA interference Structural Biology Genetics Gene silencing Animals Humans Nucleotide ATP Binding Cassette Transporter Subfamily B Member 1 Gene Silencing RNA Small Interfering Molecular Biology chemistry.chemical_classification Chemistry Nucleotides HEK 293 cells 2′-O-Methyl modification Cell Biology Molecular biology Cell biology HEK293 Cells Duplex (building) siRNA Thermodynamics |
| Zdroj: | FEBS letters. 585(14) |
| ISSN: | 1873-3468 |
| Popis: | The thermodynamic properties of siRNA duplexes are important for their silencing activity. siRNAs with high thermodynamic stability of both the central part of the duplex and in the whole, usually display low silencing activity. Destabilization of the central part of the siRNA duplex could increase its silencing activity. However, mismatches located in the central part of the duplex could substantially decrease the amount of RNAi efficacy, hindering active RISC formation and function. In this study, we examined the impact of duplex destabilization by nucleotide substitutions in the central part (7–10nt counting from the 5′-end of the antisense strand) of the nuclease-resistant siRNA on its silencing activity. |
| Databáze: | OpenAIRE |
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