Analysis of Viral Diversity in Relation to the Recency of HIV-1C Infection in Botswana
| Autor: | Vladimir Novitsky, Tulio de Oliveira, Simani Gaseitsiwe, Rosemary Musonda, Max Essex, Sikhulile Moyo, Alain Vandormael, Frank Tanser, Eduan Wilkinson, Susan Engelbrecht, Kenanao P. Kotokwe |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male RNA viruses Epidemiology Human immunodeficiency virus (HIV) lcsh:Medicine HIV Infections HIV Antibodies medicine.disease_cause Pathology and Laboratory Medicine Biochemistry Serology Medical microbiology Immunodeficiency Viruses HIV Seropositivity Genome Sequencing lcsh:Science media_common Multidisciplinary Botswana Middle Aged Viral Load Hiv seropositivity 3. Good health Medical Microbiology HIV epidemiology Viral Pathogens Lentivirus Viruses Infectious diseases Female Pathogens Viral load Research Article Adult medicine.medical_specialty media_common.quotation_subject Viral diseases Biology Research and Analysis Methods Microbiology 03 medical and health sciences Virology Retroviruses medicine Humans Molecular Biology Techniques Sequencing Techniques Microbial Pathogens Molecular Biology Medicine and health sciences lcsh:R Hiv epidemiology Organisms Genetic Variation Biology and Life Sciences HIV biology.organism_classification 030104 developmental biology HIV-1 lcsh:Q Biomarkers Viral Transmission and Infection Diversity (politics) |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 8, p e0160649 (2016) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND:Cross-sectional, biomarker methods to determine HIV infection recency present a promising and cost-effective alternative to the repeated testing of uninfected individuals. We evaluate a viral-based assay that uses a measure of pairwise distances (PwD) to identify HIV infection recency, and compare its performance with two serologic incidence assays, BED and LAg. In addition, we assess whether combination BED plus PwD or LAg plus PwD screening can improve predictive accuracy by reducing the likelihood of a false-recent result. METHODS:The data comes from 854 time-points and 42 participants enrolled in a primary HIV-1C infection study in Botswana. Time points after treatment initiation or with evidence of multiplicity of infection were excluded from the final analysis. PwD was calculated from quasispecies generated using single genome amplification and sequencing. We evaluated the ability of PwD to correctly classify HIV infection recency within |
| Databáze: | OpenAIRE |
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