Clinical isolates of Escherichia coli solely resistant to mecillinam: prevalence and epidemiology
Autor: | Hervé Delacour, Dominique De Briel, Christine Bigaillon, Sarah Bugier, Philippe Weber, Sébastien Larréché, Aurore Bousquet, Audrey Mérens, Eric Valade |
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Rok vydání: | 2018 |
Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) Genotype 030106 microbiology Anti-Infective Agents Urinary Drug resistance Urine Biology medicine.disease_cause Polymerase Chain Reaction beta-Lactam Resistance Microbiology 03 medical and health sciences Antibiotic resistance Bacterial Proteins Escherichia coli Prevalence medicine Humans Pharmacology (medical) Mecillinam Escherichia coli Infections Aged Aged 80 and over Antiinfective agent Molecular epidemiology Amdinocillin General Medicine biology.organism_classification Infectious Diseases Mutation Urinary Tract Infections Multilocus sequence typing Female Bacteria Multilocus Sequence Typing medicine.drug |
Zdroj: | International Journal of Antimicrobial Agents. 51:493-497 |
ISSN: | 0924-8579 |
Popis: | In routine susceptibility testing of Gram-negative bacteria, a particular resistance phenotype was observed: an Escherichia coli isolate from a urine sample exhibited resistance solely to mecillinam (MEC) but was fully susceptible to other β-lactam antibiotics (MEC-R-BL-S). The objectives as this study were to determine the prevalence of this phenotype and to describe the phenotype, molecular epidemiology and genetic background. Between 1 January 2014 and 31 January 2016, MEC-R-BL-S E. coli isolates from urine were collected and genes previously reported as mostly involved in MEC resistance were analysed. The genetic relatedness among isolates was investigated by repetitive element sequence-based PCR (rep-PCR) and multilocus sequence typing (MLST). Ten MEC-R-BL-S isolates were collected, accounting for 0.4% (10/2547) of all E. coli obtained from urine samples, 0.9% (10/1135) of ampicillin-susceptible E. coli isolates and 9.6% (10/104) of MEC-R E. coli isolates. The isolates appeared as small colonies with round morphology and had impaired fitness. The isolates were not clonal and belonged to various extraintestinal or commensal E. coli phylogroups. Mutations in the cysB gene were evidenced in all clinical isolates. In conclusion, microbiologists should be aware of these isolates with a particular susceptibility phenotype, which is not due to error in disk diffusion but is a real non-enzymatic antibiotic resistance pattern. |
Databáze: | OpenAIRE |
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