Lectinophagocytosis of encapsulated Klebsiella pneumoniae mediated by surface lectins of guinea pig alveolar macrophages and human monocyte-derived macrophages
| Autor: | G. G. S. Dutton, S. Markowitz, Yona Keisari, N. Sharon, Abed Athamna, Itzhak Ofek |
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| Rok vydání: | 1991 |
| Předmět: |
Phagocytosis
Guinea Pigs Molecular Sequence Data Immunology Mannose Polysaccharide Microbiology Bacterial Adhesion Monocytes chemistry.chemical_compound Lectins medicine Animals Humans Macrophage Cells Cultured Mannan chemistry.chemical_classification biology Macrophages Polysaccharides Bacterial Lectin biology.organism_classification Enterobacteriaceae Pulmonary Alveoli Klebsiella pneumoniae Infectious Diseases medicine.anatomical_structure Carbohydrate Sequence chemistry biology.protein Parasitology Rabbits Pulmonary alveolus Research Article |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.59.5.1673-1682.1991 |
| Popis: | Macrophages express a mannose/N-acetylglucosamine-specific lectin which serves as a receptor for nonopsonic phagocytosis of mannose-coated particles. We have examined the binding to guinea pig alveolar macrophages in a serum-free medium of 16 Klebsiella pneumoniae serotypes and of the capsular polysaccharides isolated from 7 of these serotypes. Only five polysaccharides containing the repeating sequence Man alpha 2/3Man or L-Rha alpha 2/3-L-Rha bound to the macrophages. Of the 11 bacterial serotypes expressing such disaccharides in their capsular polysaccharides, 7 bound efficiently, 2 bound poorly, and 2 did not bind at all. No binding occurred with five serotypes lacking these disaccharides. Binding of the bacteria was inhibited by homologous and heterologous capsular polysaccharides that contain the disaccharide sequences, by mannan, and by (Man)25BSA (where BSA is bovine serum albumin). Man alpha 2/3Man-containing oligosaccharides were potent inhibitors compared with monosaccharides. Binding was dependent on Ca2+, modulated by cultivating the macrophages on mannan-coated surfaces, and increased in human monocyte-derived macrophages compared with monocytes. The bulk of the bacteria bound to the macrophages was internalized and killed. The data taken together suggest that Klebsiella pneumoniae cells undergo lectinophagocytosis mediated by capsular disaccharides recognized by the mannose/N-acetylglucosamine-specific lectin of macrophages. This may enhance clearance of the organisms from the serum-poor environment of the lung. |
| Databáze: | OpenAIRE |
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