Low- and high-risk human papillomavirus genotype infections in intra-anal warts in HIV-positive men who have sex with men
| Autor: | Jan M. Prins, Samuel Beck, M.N.C. de Koning, H. A. M. van den Munckhof, M L Siegenbeek van Heukelom, Edyta C. Pirog, Olivier Richel, M.M. van de Sandt, Koen D. Quint, H J C de Vries |
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| Přispěvatelé: | AII - Amsterdam institute for Infection and Immunity, APH - Amsterdam Public Health, Graduate School, Dermatology, Other departments, Infectious diseases |
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male medicine.medical_specialty Genotype Human immunodeficiency virus (HIV) Dermatology medicine.disease_cause law.invention Men who have sex with men 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine law Risk Factors HIV Seropositivity medicine Humans Papillomaviridae Human papillomavirus Homosexuality Male Polymerase chain reaction Gynecology biology Carcinoma in situ Papillomavirus Infections virus diseases Middle Aged medicine.disease biology.organism_classification Anus Neoplasms Condylomata Acuminata 030220 oncology & carcinogenesis Anal warts DNA Viral Carcinoma in Situ |
| Zdroj: | British Journal of Dermatology, 175(4), 735-743 British journal of dermatology, 175(4), 735-743. Wiley-Blackwell |
| ISSN: | 1365-2133 0007-0963 |
| Popis: | Background Anogenital warts are often presumed to represent nondysplastic or low-grade anal intraepithelial neoplasia (LGAIN). We previously demonstrated that up to 20% of intra-anal warts in HIV-positive men who have sex with men (MSM) contain regions of high-grade AIN (HGAIN). Objectives To determine the causative human papillomavirus (HPV) types of low- and high- grade dysplastic areas in warts from HIV-positive MSM. Methods A total of 42 intra-anal warts from 41 HIV-positive MSM were graded as nondysplastic, LGAIN or HGAIN. Whole-tissue sections (WTS) were analysed with the SPF10 polymerase chain reaction/LiPA25 HPV genotyping system. If the WTS contained multiple HPV types, dysplastic regions were isolated by laser capture microdissection (LCM) for HPV genotyping. Results Overall, 38 of 42 (91%) WTS tested positive for HPV DNA. Of these, 23 (61%) contained a single HPV type and 15 (39%) contained multiple HPV types. All LCM-selected regions contained no more than one HPV type. Ten of 42 (24%) WTS contained HGAIN disease, of which six (60%) were associated with a high-risk HPV (hrHPV) genotype. Twenty-three of 42 WTS contained LGAIN disease, of which two (9%) were associated with hrHPV. AIN lesions containing hrHPV types were identified using p16 staining. Conclusions LGAIN lesions can be caused by high-risk HPV genotypes and vice versa. We therefore recommend routine follow-up and treatment of all dysplastic intra-anal warts for HIV-positive MSM. |
| Databáze: | OpenAIRE |
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