Expression analysis of the AtMLO Gene Family Encoding Plant-Specific Seven-Transmembrane Domain Proteins
| Autor: | Paul Schulze-Lefert, Matthew Pulley, Ming Jing Wu, H. Andreas Hartmann, Jin-Gui Chen, Erin J. Friedman, Alan M. Jones, Ralph Panstruga, Zhongying Chen |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
In silico
Locus (genetics) Plant Science Biology Genes Plant Gene Expression Regulation Plant Arabidopsis Heterotrimeric G protein Gene expression Genetics Gene family RNA Messenger Gene Phylogeny Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Membrane Proteins food and beverages General Medicine Microarray Analysis biology.organism_classification Heterotrimeric GTP-Binding Proteins Protein Subunits Transmembrane domain Multigene Family Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 60:583-597 |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | The Arabidopsis (Arabidopsis thaliana) genome contains 15 genes encoding protein homologs of the barley mildew resistance locus o (MLO) protein biochemically shown to have a seven-transmembrane domain topology and localize to the plasma membrane. Towards elucidating the functions of MLOs, the largest family of seven-transmembrane domain proteins specific to plants, we comprehensively determined AtMLO gene expression patterns by a combination of experimental and in silico studies. Experimentation comprised analyses of transgenic Arabidopsis lines bearing promoter::Beta-glucuronidase (GUS) transcriptional fusions as well as semi-quantitative determination of transcripts by reverse transcription coupled to polymerase chain reaction (RT-PCR). These results were combined with information extracted from public gene profiling databases, and compared to the expression patterns of genes encoding the heterotrimeric G-protein subunits. We found that each AtMLO gene has a unique expression pattern and is regulated differently by a variety of biotic and/or abiotic stimuli, suggesting that AtMLO proteins function in diverse developmental and response processes. The expression of several phylogenetically closely-related AtMLO genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli, suggesting functional redundancy, co-function, or antagonistic function(s). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink