Effect of Cooling on Responses of Isolated Human Airways to Pharmacologic and Electrical Stimulation
| Autor: | Johan C. de Jongste, Roberto C. Jongejan, K.F. Kerrebijn, R. C. Raatgeep, Ivan L. Bonta |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine medicine.medical_specialty Contraction (grammar) medicine.drug_class Timolol Stimulation In Vitro Techniques Epithelium chemistry.chemical_compound Internal medicine medicine Humans Sympathomimetics Lung Methacholine Chloride Aged business.industry Middle Aged respiratory system Receptor antagonist Electric Stimulation Stimulation Chemical Cold Temperature Endocrinology chemistry Cholinergic Female SRS-A Bronchoconstriction Methacholine medicine.symptom business Histamine medicine.drug |
| Zdroj: | American Review of Respiratory Disease. 143:369-374 |
| ISSN: | 0003-0805 |
| DOI: | 10.1164/ajrccm/143.2.369 |
| Popis: | We studied the effect of cooling on the responses of isolated human airways to the beta-agonist isoproterenol, the alpha/beta-agonist norepinephrine in the presence of the beta-blocker timolol, methacholine, leukotriene C4 (LTC4), and histamine. In addition, the effect of cooling on baseline airway tone and responses to electric field stimulation (EFS) was studied. At 27 degrees C the sensitivity (-logEC50) and maximal response to isoproterenol were unchanged. No measurable response was found to alpha-adrenergic stimulation with norepinephrine + timolol either before or during cooling. At 27 degrees C and 21 degrees C the sensitivity and maximal contraction to methacholine and LTC4 as well as the contraction to a single dose of histamine were reduced. Cooling diminished baseline airway tone. EFS produced a rapid cholinergic contraction followed by a deflection below baseline and a sustained noncholinergic contractile response, which was substantially reduced by the LTC4/D4 receptor antagonist FPL 55712 (11.5 microM) at all three temperatures. Cooling decreased the cholinergic response to EFS and increased the sensitivity to EFS-induced relaxation. In contrast, the sustained noncholinergic contractile response to EFS was not changed, suggesting that cooling facilitates the synthesis of LTC4/D4 that follows EFS and/or inhibits its inactivation. We conclude that in nonasthmatic, isolated human airways slow cooling of the airway wall down to 21 degrees C does not cause bronchoconstriction and does not increase the responsiveness to contractile or relaxing agonists. However, cooling increases the sensitivity to EFS-induced relaxation and might facilitate the accumulation of leukotriene C4/D4 in the airway wall.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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