Cytotoxic and Apoptotic Functions of Licofelone on Rat Glioma Cells
| Autor: | Pinar Oztopcu-Vatan, Gökhan Kuş, Ruhi Uyar, Selda Kabadere |
|---|---|
| Přispěvatelé: | Anadolu Üniversitesi, Açıköğretim Fakültesi, Sağlık Yönetimi, Kuş, Gökhan |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Glioma-Licofelone
Apoptosis General Biochemistry Genetics and Molecular Biology Flow cytometry chemistry.chemical_compound Cell Line Tumor Glioma medicine Animals Colchicine Cytotoxic T cell Pyrroles Caspase General Environmental Science Arachidonic Acid medicine.diagnostic_test biology Caspase 3 Flow Cytometry medicine.disease Caspase 9 Rats Cell biology Neurology chemistry Antiproliferative Effects biology.protein Cancer research Cyclooxygenase Drug Screening Assays Antitumor Licofelone |
| Popis: | WOS: 000327733100004 PubMed ID: 24275590 Gliomas are the largest group of central nervous system tumors and despite of clinical treatments death rate is very high. Inhibition of both cyclooxygenase and lipoxygenase pathways that take role in arachidonic acid metabolism prevents cancer development and induces apoptosis. One of the most promising compounds that blocks both of these pathways is licofelone. Using colchicine and 5-fluorouracil as positive controls, we questioned whether licofelone affects the survival of rat glioma cell line (C6) and induces apoptosis in vitro. After growing the cells in culture, we determined viability with MT, apoptosis with flow cytometry and activity of caspase enzymes with real time PCR. All used doses of colchicine and 5-fluorouracil were cytotoxic and reduced the number of surviving C6 cells as much as 44% and 60%, respectively. Comparing to the control, treatments with 10, 50 and 100 mu M licofelone for 24 or 48 h did not influence C6 survival, however, 150, 200 and 250 mu M licofelone reduced the number of living cells by 58, 88 and 93%, respectively, and induced apoptosis of C6 cells in a dose and time dependent manner. Licofelone did not change the level of caspase-9, but increased the level of caspase-3. Comparing with 5-fluorouracil and colchicine, the present study reveals for the first time the possibility that licofelone possesses a strong dose and time dependent antiproliferative and proapoptotic properties on glioma cells. Eskisehir Osmangazi University Scientific Research Projects Committee [201011017] This study was supported by a grant (number: 201011017) from Eskisehir Osmangazi University Scientific Research Projects Committee. These results were presented in part at the Congress of FEBS organized with the Turkish Society of Physiological Sciences, September, 2011, Istanbul, Turkey. |
| Databáze: | OpenAIRE |
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