Time-temperature-resolved functional and structural changes of phycocyanin extracted from Arthrospira platensis/Spirulina
| Autor: | Michael Diener, Tom Hostettler, Teresa Demuth, Lukas Böcker, Jozef Adamcik, Laura Nyström, Kai Reineke, Alexander Mathys, Severin Eder, Elena Leeb |
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| Rok vydání: | 2020 |
| Předmět: |
Time Factors
Color macromolecular substances Raw material 01 natural sciences Analytical Chemistry Pigment 0404 agricultural biotechnology Protein structure Phycocyanin Spirulina Denaturation (biochemistry) Spirulina (genus) Allophycocyanin Chromatography biology Chemistry Circular Dichroism 010401 analytical chemistry Temperature 04 agricultural and veterinary sciences General Medicine Chromophore biology.organism_classification 040401 food science 0104 chemical sciences visual_art visual_art.visual_art_medium Food Science |
| Zdroj: | Food Chemistry. 316:126374 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2020.126374 |
| Popis: | Arthrospira platensis, commonly known as Spirulina, gains increasing importance as alternative protein source for food production and biotechnological systems. A promising area is functional high-value algae extracts, rich in phycocyanin, a protein-pigment complex derived from A. platensis. This complex has proven functionality as the only natural blue colorant, fluorescent marker and therapeutic agent. The structure-function relationship is heat sensitive, making thermal processing in its production and its subsequent application a crucial aspect. In continuous high-temperature short-time treatments, it was shown how a purified phycocyanin (mixture of allophycocyanin and c-phycocyanin) disassembled and denatured between 50 and 70 °C. Three characteristic transition temperatures were allocated to specific quaternary aggregates. In contrast to sequential chemical denaturation, phycocyanin’s chromophore and protein structure were simultaneously affected by thermal processing. Through a functionality assessment, the findings help optimize the efficiency of raw material usage by defining a processing window, enabling targeted process control resulting in desired product properties. |
| Databáze: | OpenAIRE |
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