Rapid, Effective, and Versatile Extraction of Gluten in Food with Application on Different Immunological Methods
| Autor: | Jacobo Díaz, Ángela Ruiz-Carnicer, Angel Cebolla, Carolina Sousa, Verónica Segura, Alba Muñoz-Suano, Carolina Carrillo-Carrión, Isabel Comino |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
| Rok vydání: | 2021 |
| Předmět: |
Health (social science)
Gluten proteins 030309 nutrition & dietetics Plant Science lcsh:Chemical technology 01 natural sciences Health Professions (miscellaneous) Microbiology Article 03 medical and health sciences gluten-free diet Celiac disease lcsh:TP1-1185 chemistry.chemical_classification 0303 health sciences Chromatography Chemistry 010401 analytical chemistry Extraction (chemistry) Gluten extraction solution Gluten Proteins Gluten 0104 chemical sciences gluten extraction solution Solubilization Gluten-free diet Extraction methods gluten proteins celiac disease Food Science |
| Zdroj: | idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) idUS. Depósito de Investigación de la Universidad de Sevilla instname Foods Volume 10 Issue 3 Foods, Vol 10, Iss 652, p 652 (2021) |
| ISSN: | 2304-8158 |
| Popis: | One of the main concerns in gluten analysis is to achieve efficient extraction of gluten proteins. Conventional ethanol-based extraction solutions are inefficient and, because of this, it is necessary to use reducing agents or acids for proper solubilization. The extraction recommended by CODEX Standard 118-1979 (revised 2008) utilizes Cocktail solution (patent WO 02/092633 A1). However, it is harmful with a disgusting odor and is not compatible with some immunological techniques. Here, the versatility and extraction capacity of a new Universal Gluten Extraction Solution (UGES) (patent ES 2 392 412 A1) were evaluated using different methodological conditions, food matrices, and various immunological methods. UGES includes safer compounds for both the user and the environment, and it displayed similar extraction efficiency to that of the extraction method recommended for sandwich enzyme-linked immunosorbent assay (ELISA). The extraction time was significantly reduced from 100 to 40 min, depending on the type of the sample. Furthermore, unlike the currently used solution, UGES is compatible with competitive ELISA. Ministerio de Economía y Competitividad RTC-2016-5441-1 |
| Databáze: | OpenAIRE |
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