Specific Primer Sets for RT-PCR Detection of Major RNA Viruses of Tomato Plants in Korea
| Autor: | Hongsoo Choi, Jun-Sung Shin, Yu-Ju Shin, Hae-Ryun Kwak, Jung-Heon Han, Jeong-Soo Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine viruses RT-PCR Plant Science Biology 01 natural sciences Biochemistry Virus Tomato 03 medical and health sciences lcsh:Agriculture (General) Molecular Biology fungi RNA food and beverages Virology Molecular biology lcsh:S1-972 Primer Detection 030104 developmental biology Real-time polymerase chain reaction Specific primers Primer (molecular biology) Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Research in Plant Disease, Vol 23, Iss 2, Pp 193-201 (2017) |
| ISSN: | 1598-2262 |
| DOI: | 10.5423/RPD.2017.23.2.193 |
| Popis: | Major tomato viruses in Korea are Tomato chlorosis virus (ToCV), Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), Pepper mottle virus (PepMoV), and Tomato mosaic virus (ToMV). RT-PCR conditions for the viruses were examined, especially in primer set and RT-PCR mixture. Total 46 primer sets from the unique sequence of the viruses were tested for nonspecific background products in a RT-PCR mixture without template. Among them 16 primer sets were applied to healthy tomato RNA, resulting the compatibility between RT-PCR mixture and primer set influenced RT-PCR to reduce nonspecific background products. Based on the combinations among cDNA synthesis parameters and RT-PCR mixtures, two reaction mixtures were finally selected for ToCV detection. The condition allowed to determine more specific primer sets; C029 (ToCV), C072 (TSWV), C070 (CMV), C048 (PepMoV), and C065 (ToMV). These primer sets are expected to be of use to specific detection of the major viruses in tomato plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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