Dictyostelium lacking the single atlastin homolog Sey1 shows aberrant ER architecture, proteolytic processes and expansion of the Legionella ‐containing vacuole
| Autor: | A. Leoni Swart, François Letourneur, Amanda Welin, Dario Hüsler, Virginie Molle, Bianca Striednig, Bernhard Steiner, Hubert Hilbi |
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| Přispěvatelé: | Institute of Medical Microbiology [Zurich], Universität Zürich [Zürich] = University of Zurich (UZH), LPHI - Laboratory of Pathogen Host Interactions (LPHI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Atlastin
Movement Immunology Protozoan Proteins Vacuole Endoplasmic Reticulum Microbiology Legionella pneumophila Dictyostelium discoideum GTP Phosphohydrolases 03 medical and health sciences 0302 clinical medicine Phosphatidylinositol Phosphates Virology Homeostasis Dictyostelium ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Endoplasmic reticulum biology.organism_classification Endoplasmic Reticulum Stress [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Cell biology Host-Pathogen Interactions Vacuoles Unfolded protein response Muramidase Endoplasmic Reticulum Rough 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Cellular Microbiology Cellular Microbiology, Wiley, 2021, 23 (5), ⟨10.1111/cmi.13318⟩ |
| ISSN: | 1462-5814 1462-5822 |
| Popis: | Dictyostelium discoideum Sey1 is the single ortholog of mammalian atlastin 1-3 (ATL1-3), which are large homodimeric GTPases mediating homotypic fusion of endoplasmic reticulum (ER) tubules. In this study, we generated a D. discoideum mutant strain lacking the sey1 gene and found that amoebae deleted for sey1 are enlarged, but grow and develop similarly to the parental strain. The ∆sey1 mutant amoebae showed an altered ER architecture, and the tubular ER network was partially disrupted without any major consequences for other organelles or the architecture of the secretory and endocytic pathways. Macropinocytic and phagocytic functions were preserved; however, the mutant amoebae exhibited cumulative defects in lysosomal enzymes exocytosis, intracellular proteolysis, and cell motility, resulting in impaired growth on bacterial lawns. Moreover, ∆sey1 mutant cells showed a constitutive activation of the unfolded protein response pathway (UPR), but they still readily adapted to moderate levels of ER stress, while unable to cope with prolonged stress. In D. discoideum ∆sey1 the formation of the ER-associated compartment harbouring the bacterial pathogen Legionella pneumophila was also impaired. In the mutant amoebae, the ER was less efficiently recruited to the "Legionella-containing vacuole" (LCV), the expansion of the pathogen vacuole was inhibited at early stages of infection and intracellular bacterial growth was reduced. In summary, our study establishes a role of D. discoideum Sey1 in ER architecture, proteolysis, cell motility and intracellular replication of L. pneumophila. |
| Databáze: | OpenAIRE |
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