Oral and Intraperitoneal Administration of Phosphorothioate Oligodeoxynucleotides Leads to Control ofCryptosporidium parvumInfection in Neonatal Mice
| Autor: | Fabrice Laurent, Anne-Marie Chaussé, Satoshi Uematsu, Sonia Lacroix-Lamandé, Shizuo Akira, Nelly Bernardet, Mathieu Barrier, Gaël Auray, Roselyne Mancassola |
|---|---|
| Přispěvatelé: | Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Osaka University [Osaka] |
| Rok vydání: | 2006 |
| Předmět: |
Male
[SDV]Life Sciences [q-bio] medicine.medical_treatment Administration Oral Cryptosporidiosis ADJUVANT Polymerase Chain Reaction Parasite load Mice MOTIFS 0302 clinical medicine Parasitic Sensitivity Tests Oral administration Immunology and Allergy PROTECTION IMMUNE-RESPONSES T-LYMPHOCYTES Mice Knockout Mice Inbred BALB C 0303 health sciences biology hemic and immune systems respiratory system Specific Pathogen-Free Organisms 3. Good health CPG OLIGODEOXYNUCLEOTIDES GAMMA-INTERFERON BACTERIAL-DNA Infectious Diseases Cryptosporidium parvum Cytokine Oligodeoxyribonucleotides CpG site Female Injections Intraperitoneal RNA Protozoan EXPRESSION CpG Oligodeoxynucleotide Drug Administration Schedule 03 medical and health sciences Immune system Adjuvants Immunologic medicine Animals Humans 030304 developmental biology IMMUNOCOMPETENT TLR9 biology.organism_classification Mice Inbred C57BL Disease Models Animal Animals Newborn Immunology 030215 immunology |
| Zdroj: | HAL Journal of Infectious Diseases Journal of Infectious Diseases, Oxford University Press (OUP), 2006, 193 (10), pp.1400-1407. ⟨10.1086/503748⟩ |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | International audience; Background. Neonates are particularly vulnerable to infections, in part because of the incomplete development of their immune system. Recent advances in immunostimulatory treatments based on conserved microbial components led us to assess the potential of oligodeoxynucleotides ( ODNs) for decreasing the sensitivity of neonates to Cryptosporidium parvum infection. Methods. Neonate mice were treated orally or intraperitoneally ( ip) with CpG ODNs or non-CpG ODNs 24 h before C. parvum infection, and parasite load and cytokine up-regulation were evaluated. Results. CpG ODN 1668 and non-CpG ODN 1668 administered orally, as well as CpG ODN 1668 administered ip, induced an 80%-95% decrease in intestinal parasite load 6 days after infection. Intraperitoneal and oral pretreatment with CpG ODN 1668 led to a strong initial up-regulation of cytokines and CD69 messenger RNA in the intestine and a decrease in parasite load by a Toll-like receptor 9 ( TLR9)-dependent mechanism. By contrast, oral administration of non-CpG ODN 1668 decreased parasite load by a TLR9-independent mechanism. Conclusion. The control of neonatal C. parvum infection by ip or oral administration of ODNs is feasible by 2 different mechanisms: ( 1) the well-known interaction involving CpG/TLR9, leading to the production of cytokines and lymphocyte activation, and ( 2) a new unknown mechanism that is independent of TLR9 and effective orally. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|