A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans

Autor:	R. J. Woods, J. Greally
Rok vydání:	2016
Předmět:	Microculture Tetanus business.industry Pokeweed mitogen Tuberculin chemical and pharmacologic phenomena General Medicine medicine.disease Lymphocyte Activation In vitro Antigen Lymphocyte transformation Immunology medicine Methods Antigens Mitogens business Pan-T antigens Cells Cultured
Zdroj:	Irish journal of medical science. 145(1)
ISSN:	0021-1265
Popis:	A micro method forin vitro transformation of lymphocytes (Penhaleet al., 1974) by mitogens and antigens is presented. Using a range of concentrations, satisfactory responses were obtained with small numbers of lymphocytes in the presence of the mitogens, phytohaemagglutinin-P, Concanavalin-A, Pokeweed mitogen and mumps, tetanus, tuberculin (Purified Protein Derivative) antigens. Diptheria toxoid and streptokinase/streptodornase antigens did not give satisfactory responses. The method is likely to be of particular use in studies in lymphopenic individuals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5aa15471ea6b85a2e95f683147b04d56 https://pubmed.ncbi.nlm.nih.gov/27517258 Zobrazit plný text záznamu Full text from SpringerLink