Expression of Gross Cystic Disease Fluid Protein-15/Prolactininducible Protein in Rat Salivary Glands
| Autor: | Lily Mirels, Holly J. Branin, Arthur R. Hand |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
0301 basic medicine Aging Rodent Salivary Glands Rats Sprague-Dawley Mice Peptide sequence biology Chemistry Gene Expression Regulation Developmental Immunohistochemistry Submandibular gland Parotid gland Serous fluid Prolactin-Inducible Protein medicine.anatomical_structure Female lipids (amino acids peptides and proteins) Anatomy musculoskeletal diseases medicine.medical_specialty DNA Complementary Histology Blotting Western Molecular Sequence Data 03 medical and health sciences stomatognathic system Internal medicine Complementary DNA biology.animal Biomarkers Tumor medicine Animals Humans Amino Acid Sequence Rats Wistar Saliva Apolipoproteins D Glycoproteins Base Sequence Sequence Homology Amino Acid 030102 biochemistry & molecular biology Membrane Transport Proteins Sublingual gland Blotting Northern Rats Apolipoproteins 030104 developmental biology Endocrinology Animals Newborn Carrier Proteins |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 46:1061-1071 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/002215549804600910 |
| Popis: | Gross cystic disease fluid protein-15 (GCDFP-15)/prolactin-inducible protein (PIP) is present at moderate levels in human submandibular and sublingual glands and is barely detectable in human parotid gland. The rodent homologue, PIP, has previously been identified in adult submandibular and lacrimal glands. Here we present the molecular characterization of rat PIP and show that this protein is a product of neonatal and adult rat submandibular, sublingual, and parotid glands. cDNA clones encoding rat PIP were isolated and sequenced. The deduced amino acid sequence of rat PIP shows 56% overall identity and 80% similarity with mouse PIP. By SDS-PAGE, secreted rat PIP has an apparent Mrof 17,000, with a minor proportion present as Mr20–22,000 N-glycosylated forms. PIP was localized in rat salivary glands by immunogold silver staining. PIP was identified in acinar cells of developing and mature submandibular and parotid glands and at very low levels in sublingual gland serous demilunes. Typically, rat submandibular gland secretory proteins are produced by either acinar cell progenitors (Type III cells) or mature acinar cells. The expression pattern observed for PIP is similar to that previously reported for salivary peroxidase, an important component of nonimmune mucosal defense. |
| Databáze: | OpenAIRE |
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