Actinomycin Analogues Containing Pipecolic Acid: Relationship of Structure to Biological Activity
| Autor: | Aaron J. Shatkin, Joseph V. Formica, Edward Katz |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
DNA
Bacterial Proline Microbial Physiology and Metabolism Staphylococcus Sarcina Vaccinia virus Bacillus subtilis Reoviridae Virus Replication Microbiology chemistry.chemical_compound L Cells Bacterial Proteins Escherichia coli Protein biosynthesis medicine Uridine Molecular Biology Polymerase Pipecolic acid Carbon Isotopes Dactinomycin biology DNA synthesis RNA RNA Nucleotidyltransferases Valine DNA biology.organism_classification Streptomyces Poliovirus RNA Bacterial chemistry Biochemistry Spectrophotometry Pipecolic Acids biology.protein Thymidine medicine.drug |
| Zdroj: | Journal of Bacteriology. 95:2139-2150 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.95.6.2139-2150.1968 |
| Popis: | Streptomyces antibioticus synthesizes a mixture of actinomycins which differ at the “imino acid” site of the peptide chains. In the presence of exogenous pipecolic acid, several new actinomycins were synthesized and 70% of the proline in the antibiotic mixture was replaced by the analogue. Three new antibiotics (designated Pip 1α, Pip 1β, and Pip 2) were isolated from culture filtrates, purified, and crystallized. The molar ratio of pipecolic acid to proline was: Pip 1α, 1:0; Pip 1β, 1:1; Pip 2, 2:0. These compounds inhibited the growth and cell division of gram-positive, but not gram-negative, bacteria. The relative inhibitory activity against bacteria, Escherichia coli deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase in vitro, and RNA synthesis in Bacillus subtilis and mouse L-929 cells was: actinomycin IV = Pip 1β > Pip 2 > Pip 1α. Protein synthesis in B. subtilis was less affected, and DNA synthesis was inhibited only at higher concentrations of antibiotic tested. In L cells, DNA formation was reduced less than RNA synthesis, whereas protein synthesis was not blocked under the experimental conditions employed. Kinetic studies with B. subtilis revealed that RNA synthesis was inhibited rapidly followed by an inhibition of protein synthesis. All four antibiotics markedly inhibited the replication of vaccinia virus and reovirus in tissue culture cells, but the production of poliovirus was resistant to the antibiotics. These actinomycins bind to DNA, resulting in an elevation of its T m and a decrease in the peak extinction of the actinomycins. The mode of action, as well as the structure-activity relationships among the actinomycins, are discussed relative to a previously proposed model of binding. |
| Databáze: | OpenAIRE |
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