The Daisho Peptides Mediate Drosophila Defense Against a Subset of Filamentous Fungi

Autor: Lianne B. Cohen, Yangyang Xu, Steven A. Wasserman, Scott A. Lindsay, Samuel J. H. Lin
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
lcsh:Immunologic diseases. Allergy
Antimicrobial peptides
Immunology
Hyphae
Genetically Modified
Candida glabrata
toll
Microbiology
Animals
Genetically Modified
humoral
03 medical and health sciences
Gene Knockout Techniques
0302 clinical medicine
Immune system
Fusarium
Hemolymph
Fusarium oxysporum
Enterococcus faecalis
Genetics
Immunology and Allergy
CRISPR
Animals
Drosophila Proteins
Innate
innate immunity
Original Research
Innate immune system
biology
Effector
fungi
Immunity
biology.organism_classification
Immunity
Innate
030104 developmental biology
Drosophila melanogaster
Emerging Infectious Diseases
Infectious Diseases
Medical Microbiology
Host-Pathogen Interactions
Drosophila
CRISPR-Cas Systems
Infection
lcsh:RC581-607
antifungal
030215 immunology
Antimicrobial Cationic Peptides
Signal Transduction
Zdroj: Frontiers in Immunology, Vol 11 (2020)
Frontiers in Immunology
ISSN: 1664-3224
DOI: 10.3389/fimmu.2020.00009/full
Popis: Fungal infections, widespread throughout the world, affect a broad range of life forms, including agriculturally relevant plants, humans, and insects. In defending against fungal infections, the fruit fly Drosophila melanogaster employs the Toll pathway to induce a large number of immune peptides. Some have been investigated, such as the antimicrobial peptides (AMPs) and Bomanins (Boms); many, however, remain uncharacterized. Here, we examine the role in innate immunity of two related peptides, Daisho1 and Daisho2 (formerly IM4 and IM14, respectively), found in hemolymph following Toll pathway activation. By generating a CRISPR/Cas9 knockout of both genes, Δdaisho, we find that the Daisho peptides are required for defense against a subset of filamentous fungi, including Fusarium oxysporum, but not other Toll-inducible pathogens, such as Enterococcus faecalis and Candida glabrata. Analysis of null alleles and transgenes revealed that the two daisho genes are each required for defense, although their functions partially overlap. Generating and assaying a genomic epitope-tagged Daisho2 construct, we detected interaction in vitro of Daisho2 peptide in hemolymph with the hyphae of F. oxysporum. Together, these results identify the Daisho peptides as a new class of innate immune effectors with humoral activity against a select set of filamentous fungi.
Databáze: OpenAIRE
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