Cellular magnetic resonance imaging: in vivo tracking of gastric cancer cells and detecting of lymph node metastases using microparticles of iron oxide in mice
| Autor: | Jianxi Zhao, Jian Chen, Rong Cai, Chen Guo, Xiangru Wu, Gang Ren, Huali Li, Ting Gui |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Iron oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine magnetic iron oxide particles In vivo medicine magnetic resonance imaging Lymph node Original Research lymph node metastasis medicine.diagnostic_test gastric cancer Magnetic resonance imaging In vitro Staining 030104 developmental biology Lymphatic system medicine.anatomical_structure Oncology chemistry Cancer Management and Research 030220 oncology & carcinogenesis Cancer cell |
| Zdroj: | Cancer Management and Research |
| ISSN: | 1179-1322 |
| Popis: | Jian Chen,*,1,2 Gang Ren,1 Rong Cai,3 Xiangru Wu,*,4 Ting Gui,1 Jianxi Zhao,1 Huali Li,1 Chen Guo11Department of Radiology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, People’s Republic of China; 2Department of Radiology, Children’s Hospital of Fudan University, Shanghai 201102, People’s Republic of China; 3Department of Radiotherapy, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People’s Republic of China; 4Department of Pathology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, People’s Republic of China*These authors contributed equally to this workBackground: Monitoring the fate of implanted cells over time in an experimental animal may provide a new way to track the metastatic process. Lymph node metastase is of extremely importance for the prognostic prediction of gastric carcinoma. The aim of this study was to assess the feasibility of magnetic resonance imaging (MRI), using micron-sized superparamagnetic iron oxide particles (MPIO), for monitoring of the fate of gastric cancer cells and detecting the migration of gastric cancer cells through the lymphatic system in a mouse model.Methods: SGC-7901 gastric cancer cells were labeled with green fluorescent MPIO. The cells were monitored in vitro at multiple time points by staining for iron-labeled cells and by flow cytometric detection of the fluorescent MPIO. MPIO-labeled cells were implanted subcutaneously into nude mice, and cellular MRI was performed at different time points until 35days postinjection.Results: The potential for retention of the iron particles in vitro was evaluated. Our results showed that the labeling and uptake efficiency of MPIO reached 90.0% after 24hrs of incubation, and a small percentage of cells that retained MPIO could be examined until 16days after labeling. In vivo MRI-based tracking over several weeks in mice revealed regions of signal loss in the primary tumors for up to 5weeks. Furthermore, small regions of signal void were detected in images of the inguinal lymph nodes in three mice at day 28 postinjection or later, and histological assays confirmed the presence of iron-labeled cancer cells.Conclusion: This study supports MPIO-based cell tracking is a useful tool for monitoring the fate of gastric cancer cells in mice over time, which may facilitate progress in understanding the mechanisms of early regional lymph node micrometastases.Keywords: magnetic resonance imaging, magnetic iron oxide particles, lymph node metastasis, gastric cancer |
| Databáze: | OpenAIRE |
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